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Histopaque® Density Gradient Media Troubleshooting Guide
WEBDensity Gradient Media Troubleshooting Guide. The following recommendations for troubleshooting the use of Histopaque and ACCUSPIN™ in cell separation techniques have been compiled by our Technical Support scientists based on observations and …
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Isolation of lymphocytes from whole blood
WEB2 days ago · The separation medium, HISTOPAQUE-1077, is an aqueous solution of a high molecular weight polysaccharide and sodium diatrizoate, aniodinated nonionic compound, adjusted to a density of 1.077 ± 0.001. This medium facilitates rapid recovery of viable lymphocytes from small volumes of blood.
Filtration is a time-efficient option to Histopaque, providing good ...
WEBJan 19, 2014 · In this study we aimed to compare the quality of islets obtained by an established method of islet purification, Histopaque, with an appealing alternative one, filtration, although some modifications of the original protocol were needed.
- Author: Miriam Ramírez-Domínguez, Luis Castaño
- DOI: 10.1007/s10616-014-9690-7
- Publish Year: 2015
- Publication: Cytotechnology. 2015 Mar; 67(2): 199-206.
- Studies of Histopaque Method
Histopaque -1077, Sterile-filtered and endotoxin-tested
WEBHistopaque-1077 is a sterile, endotoxin tested, ready-to-use medium. The Histopaque composition includes polysucrose and sodium diatrizoate, adjusted to a density of 1.077g/mL. This ready-to-use medium facilitates rapid recovery of viable lymphocytes and other mononuclear cells from small volumes of whole blood or bone marrow.
Accuspin™ System with Histopaque®-1077: Comprehensive Cell …
WEBApr 12, 2022 · See how the Accuspin™ System with Histopaque®-1077 is an efficient method for separating lymphocytes and other nuclear cells from whole blood or bone marrow.
Optimization of methods for the accurate characterization of …
WEBMar 7, 2022 · By comparing eight methods of neutrophil characterization, we demonstrate that the level of neutrophil activation and degranulation is associated with specific experimental conditions and the ...
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Effective high-throughput isolation of fully human antibodies
WEBOct 7, 2019 · Perform PBMC isolation by standard density gradient centrifugation using Histopaque separation medium and Leucosep cell tubes according to the manufacturer’s protocol. Pause point Cells can be ...
Isolation, Purification and Labeling of Mouse Bone Marrow …
WEBJul 10, 2013 · To date, several methods have been described for the isolation of neutrophils such as density gradient centrifugation of human blood and mouse blood or bone marrow 8,9 , positive or negative immunomagnetic enrichment of neutrophils from mouse blood or bone marrow 10,11 , and harvesting of neutrophils from the peritoneal cavity of mice …
WEBIn general, the procedures employed mixtures of polysaccharide and a radiopaque contrast medium. HISTOPAQUE 1083 is a solution of polysucrose and sodium diatrizoate, adjusted to a density of 1.083 g/ml. This medium facilitates rapid recovery of viable mononuclear cells from small volumes of blood. ACCUSPIN TUBES.
WEBCentrifuge at 400 x g for 35 minutes at room temperature. During this step, the granulocytes, platelets and RBCs pellet to the bottom of the tube and the PBMC float over the Ficoll-Paque PLUS or Histopaque-1077. Carefully aspirate the PBMC from the Ficoll-plasma interface. Wash the PBMC with PBS at 300 x g at 4 C twice.
Comparison of methods to isolate peripheral blood
WEBJan 1, 2023 · Methods were compared to a traditional Histopaque-1077 separation method historically used in the laboratory and assessed for ability to recover subpopulations of lymphocytes and monocytes by flow cytometric analyses.
Gamma-H2AX biodosimetry for use in large scale radiation …
WEBMar 6, 2014 · Routine histopaque method. The histopaque method has been described in detail by Rothkamm et al. (2013b). In brief, 2 ml of whole blood was mixed 50:50 with PBS and layered onto 4 ml Histopaque 1077 (Sigma, UK) in 15 ml conical centrifuge tubes.
Filtration is a time-efficient option to Histopaque, providing good ...
WEBFor many years the method used worldwide for islet purification in both rodent and human islet isolation has been Ficoll-based density gradients, such as Histopaque. However, it is difficult to purify islets in laboratories with staff limitations when large scale isolations are …
35 questions with answers in HISTOPAQUE | Science topic
WEBJun 14, 2023 · Review and cite HISTOPAQUE protocol, troubleshooting and other methodology information | Contact experts in HISTOPAQUE to get answers
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WEBHistopaque ® Density Gradient Media Troubleshooting Guide. Mark Frei. The following recommendations for troubleshooting the use of Histopaque and ACCUSPIN™ in cell separation techniques have been compiled by our Technical Support scientists based on observations and experience from resolving customer problems.
Comparison of methods to isolate peripheral blood mononuclear …
WEBOur laboratory method uses Histopaque-1077 density gradient in standard conical tubes and this was compared with isolation of cells using SepMate™ tubes, a novel conical tube containing an insert to separate the density gradient. Multiple experiments were performed to optimize the SepMate™ tubes for use with cattle blood.
Use of Histopaque for isolating mononuclear cells from rabbit …
WEBSep 24, 1987 · histopaque. This paper describes the use of Histopaque (Hp) density gradient medium and its advantage for separating mononuclear cells (mnc) from rabbit blood. Hp solutions of density (d) = 1.083 g/ml, 1.119 g/ml and 1:1 mixture of these solutions (final d = 1.103 g/ml) were compared to Ficoll-Paque (Fp, d = 1. …
Comparison of the Histopaque-1119 method with the Plasmagel …
WEBHistopaque-1119 (Sigma Chemical Co., St. Louis, Mo.) and Plasmagel (Cellular Products, Inc., Buffalo, N.Y.) were compared as density gradient separation reagents for the separation of polymorphonuclear leukocytes and mononuclear cells from blood from the isolation of cytomegalovirus (CMV).
WEBHistopaque -1119 is a sterile, endotoxin tested solution of polysucrose and sodium diatrizoate, adjusted to a density of 1.119 g/mL. This ready-to-use medium, when used in conjunction with Histopaque-1077, facilitates the rapid recovery of viable mononuclear cells and granulocytes from small volumes of whole blood.
Comparison of the methods for platelet rich plasma preparation in ...
WEBAug 18, 2018 · This study demonstrated that separated centrifugation using histopaque was the best method for platelet rich plasma preparation because of the proper amount of platelets and the separation of red blood cells from platelet rich plasma.
Ficoll-Paque PLUS density gradient media | Cytiva
WEBA recognized worldwide standard sterile medium designed for isolating human lymphocytes for <i>in vitro</i> studies.
Recommended Standard Method for Isolating Mononuclear Cells
WEBCell separation using Ficoll-Paque products can be carried out over a wide range of blood sample volumes. With its high yield, this method can be adapted to the processing of very small amounts of blood, such as may be obtained from children. For maximum reproducibility of separation it is recommended that a standardized procedure be used.
Histopaque -1077, Sterile-filtered and endotoxin-tested
WEBisolation of breast cancer circulating tumor cells (CTCs) from the whole blood. Histopaque-1077 is used in conjunction with Histopaque-1119 as it facilitates the rapid recovery of viable mononuclear cells and granulocytes from small volumes of whole blood.
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