You can perform cryosectioning on both your formalin-fixed and fresh tissue samples. However, formalin fixation better preserves the antigens within your tissue. Briefly, tiss… See more

Place your prepared tissue block within the cryostat chamber (Figure 1) for 30-60 minutes prior to beginning your sectioning, to allow the tissue to acclimate to -200C. You shoul… See more

This sounds silly but if you have never sectioned this is a real consideration. Sectioning is a bit like “patting your head and rubbing your stomach at the same time”. It tak… See more

You will need to clean the cryostat after every session, and likely a few times during. But never clean components inside the chamber with water! If you do the component… See more