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The following protocols describe the immune DNA-PAINT staining procedure for adherent cells expressing a GFP-tagged protein grown on glass coverslips. Version (1) and (2) Docking …

Here, we present a protocol for the key aspects of the DNA-PAINT framework for both novice and expert users. This protocol describes the creation of DNA origami test samples, in situ sample …

This protocol describes the creation of DNA origami test samples, in situ sample preparation, multiplexed data acquisition, data simulation, super-resolution image reconstruction and post …

DNA-PAINT uses base-pairing between short fluorescently labeled DNA oligonucleotides . A target is labeled with a short DNA docking strand while the complementary, fluorescently …

May 14, 2024 · DNA-PAINT is a super-resolution microscopy technique that involves targeting proteins of interest with antibodies that are linked to short DNA sequences called docking …

Nov 25, 2022 · Here, we provide a detailed, step-by-step guide for fast and multiplexed DNA-PAINT-ERS imaging of fixed and immunostained cells grown on glass substrates as adherent …

Oct 18, 2024 · In this study, we introduce a new labeling procedure, called Shielded DNA-PAINT, that greatly reduces the levels of non-specific interactions within cells when preparing samples …

Here we describe the protocol to conduct Repeat DNA-PAINT which uses docking domains repeated up to 10x on the same strand, enabling an order of magnitude decrease in imager …

DNA-PAINT can be applied to a multitude of in vitro and cellular applications by linking docking strands to antibodies. Here, we present a protocol for the key aspects of the DNA-PAINT …

Here, we present a protocol for the key aspects of the DNA-PAINT framework for both novice and expert users. This protocol describes the creation of DNA origami test samples, in situ sample …

Here, we provide a detailed, step-by-step guide for fast and multiplexed DNA-PAINT-ERS imaging of fixed and immunostained cells grown on glass substrates as adherent monolayers.

Here, we report a step-by-step protocol on how to utilize DNA-PAINT to perform super-resolution imaging of ER-phagy receptors in fixed cells. In addition, we provide a detailed explanation of …

In a wonderfully detailed protocol on how to use DNA-PAINT and super-resolution microscopy (Schnitzbauer et al., 2017), the authors show how to get novices and expert users using not …

Nov 4, 2016 · DNA-PAINT (DNA-based point accumulation for imaging in nanoscale topography) is a super-resolution method that exploits programmable transient hybridization between short …

DNA-PAINT-ERS uses existing DS-IS pairs and workflows with minor modifications that can be easily implemented. Below we provide detailed, step-by-step instructions for performing single- …

Mar 7, 2023 · This review provides a comprehensive summary of the repertoire of labelling probes used for DNA-PAINT in cells and the strategies implemented to chemically modify them with a …

3 days ago · Proper handling protocols and DNA contamination prevention remain vital for maintaining sample integrity throughout the forensic science DNA analysis process. ... Arson …

15 hours ago · Finally, the DNA backbone is sealed, and the damaged DNA is restored back to health. Mapping of XP and XP/CS disease mutations onto the PInC community structure. …