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  1. Nature
    https://www.nature.com/articles/s41594-020-0380-1

    FANCD2–FANCI is a clamp stabilized on DNA by

    • Based on previously described ubiquitination assays8,28, we set up a large-scale in vitro reconstitution of the FA core complex−mediated ubiquitination of D2–I. In a total volume of 400 µl, the reaction contained 7… See more

    Cloning, Expression and Purification

    cDNAs encoding full-length Gallus gallus FANCI and FANCD2 were synthesized (GeneArt) and cloned into pACEBac1. The individual genes were amplified by PCR and cloned … See more

    Nature
    Electrophoretic Mobility Shift Assays

    Fluorescently labeled DNA (6FAM-labeled or Alexa647-labeled on the 3ʹ end, purchased from … See more

    Nature
    Gel Filtration Assays

    A volume of 300 µl of purified D2–I, FANCD2 and FANCI at 1 µM were sequentially run on a Superdex 200 10/300 column (GE Healthcare Life Sciences) equilibrate… See more

    Nature
    Protein Binding Assays

    We used purified StrepII-tagged FANCD2 and His-tagged FANCI to probe their interaction. In a total volume of 50 µl per reaction, FANCD2:FANCI at molar ratios 1:0, 1:0.5, 1… See more

    Nature
    Electron Microscopy and Image Processing

    Three microliters of ~1 µM ubD2–I, D2–I or D2 complex was blotted on plasma-cleaned UltraAufoil R1.2/1.3 grids45 (Quantifoil) for 3–4.5 s and plunged into liquid ethane using a Vitr… See more

    Nature
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    Table of Contents
    Cloning, Expression and Purification
    Electrophoretic Mobility Shift Assays
    Gel Filtration Assays
    Protein Binding Assays
    Electron Microscopy and Image Processing
     

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  2. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7067600

    FANCD2–FANCI is a clamp stabilized on DNA by …

     
  3. Nature
    https://www.nature.com/articles/s41586-024-07770-w

    FANCD2–FANCI surveys DNA and recognizes double- to single …

  4. Studies of fancd2 dimerization
    National Center for Biotechnology Information
    FANCD2–FANCI is a clamp stabilized on DNA by monoubiquitination of FANCD2 during DNA repair
    Vertebrate DNA crosslink repair excises toxic replication-blocking DNA crosslinks. Numerous factors involved in crosslink repair have been identified, and mutations in their corresponding genes cause Fanconi anemia (FA). A key step in crosslink repair
    Author: Pablo Alcón, S…
    Published: 2020/03
    DOI: 10.1038/s41594-0…
    febs.onlinelibrary.wiley.com
    Mechanism, specificity, and function of FANCD2-FANCI ubiquitination and deubiquitination
    Fanconi anemia (FA) is a rare genetic disorder caused by mutations in any of the currently 22 known FA genes. The products of these genes, along with other FA-associated proteins, participate in a biochemical pathway, known as the FA pathway. This pathway
    Author: Kimon Lemoni…
    Published: 17 June 2021
    Cited By: 12
    ResearchGate
    Mechanism, specificity, and function of FANCD2-FANCI ubiquitination and deubiquitination
    Fanconi anemia (FA) is a rare genetic disorder caused by mutations in any of the currently 22 known FA genes. The products of these genes, along with other FA‐associated proteins, participate in a biochemical pathway, known as the FA pathway. This pathway
    Author: Kimon Lemoni…
    Published: 2021/06/01
    DOI: 10.1111/febs.16077
    National Center for Biotechnology Information
    Structure of the FANCI-FANCD2 Complex: Insights into the Fanconi Anemia DNA Repair Pathway
    Fanconi Anemia is a cancer predisposition syndrome caused by defects in the repair of DNA interstrand crosslinks (ICL). Central to this pathway is the FANCI-FANCD2 (ID) complex, which is activated by DNA damage-induced phosphorylation and monoubiquitination.
    Author: Woo Joo, Guo…
    Published: 2011/07/07
    DOI: 10.1126/science.1…
    National Center for Biotechnology Information
    Structural insight into FANCI–FANCD2 monoubiquitination
    The Fanconi anemia (FA) pathway coordinates a faithful repair mechanism for DNA damage that blocks DNA replication, such as interstrand cross-links. A key step in the FA pathway is the conjugation of ubiquitin on to FANCD2 and FANCI, which is facilitated
    Author: Landing Li, Wi…
    Published: 2020/10
    Publication: Essays Bi…
    PubMed
    FANCD2 and RAD51 recombinase directly inhibit DNA2 nuclease at stalled replication forks and FANCD2 acts as a novel RAD51 mediator in strand exchange to promote genome stability
    FANCD2 protein, a key coordinator and effector of the interstrand crosslink repair pathway, is also required to prevent excessive nascent strand degradation at hydroxyurea-induced stalled forks. The RAD51 recombinase has also been implicated in regulation
    Author: Wenpeng Liu
    Published: 2023 Aug
    Keywords: National In…
    National Center for Biotechnology Information
    The DNA-damage kinase ATR activates the FANCD2-FANCI clamp by priming it for ubiquitination
    DNA interstrand crosslinks are tumor-inducing lesions that block DNA replication and transcription. When crosslinks are detected at stalled replication forks, ATR kinase phosphorylates FANCI, which stimulates monoubiquitination of the FANCD2-FANCI clamp
    Author: Tamara Sijacki…
    Published: 2022/09
    DOI: 10.1038/s41594-0…
    PubMed
    Structural insight into FANCI-FANCD2 monoubiquitination
    The Fanconi anemia (FA) pathway coordinates a faithful repair mechanism for DNA damage that blocks DNA replication, such as interstrand cross-links. A key step in the FA pathway is the conjugation of ubiquitin on to FANCD2 and FANCI, which is facilitated
    Author: Landing Li
    Published: 2020/10/26
    Keywords: DNA synth…
  5. Cell Press
    https://www.cell.com/cell-reports/fulltext/S2211-1247(16)31554-6

    The FA Core Complex Contains a Homo-dimeric Catalytic

  6. Nature
    https://www.nature.com/articles/s41594-022-00820-9

    The DNA-damage kinase ATR activates the FANCD2-FANCI …

  7. FEBS Press
    https://febs.onlinelibrary.wiley.com/doi/full/…

    Mechanism, specificity, and function of …

    WEBJun 17, 2021 · Dimonoubiquitination of the FANCI-FANCD2 (ID2) complex is a crucial step in the Fanconi anemia DNA repair pathway. This is achieved by the E2-ubiquitin-conjugating enzyme, UBE2T, and a …

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  9. bioRxiv
    https://www.biorxiv.org/content/biorxiv/early/2019/11/28/858225.full.pdf
    [PDF]

    FANCD2-FANCI is a clamp stabilized on DNA by ... - bioRxiv

  10. ResearchGate
    https://www.researchgate.net/publication/352481421...

    (PDF) Mechanism, specificity, and function of FANCD2-FANCI ...

  11. EMBO Press
    https://www.embopress.org/doi/full/10.152…

    Differential functions of FANCI and FANCD2

    WEBJun 8, 2020 · Our above results demonstrate separate functions for the two monoubiquitination events occurring on the ID2 complex. FANCD2 ubiquitination was found to modulate ID2‐dsDNA binding affinity, …

  12. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3310437

    Structure of the FANCI-FANCD2 Complex: Insights into the …

  13. Oxford Academic
    https://academic.oup.com/nar/article/45/20/11837/4559113

    FANCI and FANCD2 have common as well as independent …

  14. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7588663

    Structural insight into FANCI–FANCD2 monoubiquitination

  15. ScienceDirect
    https://www.sciencedirect.com/science/article/pii/S2211124716315546

    The FA Core Complex Contains a Homo-dimeric Catalytic …

  16. Nature
    https://www.nature.com/articles/s41594-022-00826-3

    The key to the FANCD2–FANCI lock - Nature

  17. PubMed
    https://pubmed.ncbi.nlm.nih.gov/37526271

    FANCD2 and RAD51 recombinase directly inhibit DNA2 nuclease …

  18. cell.com
    https://www.cell.com/molecular-cell/fulltext/S1097-2765(16)30713-4

    Mechanism of Ubiquitination and Deubiquitination in the Fanconi …

  19. Cell Press
    https://www.cell.com/cell-reports/fulltext/S2211-1247(14)00292-7

    FANCD2 Binds CtIP and Regulates DNA-End Resection during …

  20. PubMed
    https://pubmed.ncbi.nlm.nih.gov/32725171

    Structural insight into FANCI-FANCD2 monoubiquitination

  21. PubMed
    https://pubmed.ncbi.nlm.nih.gov/27986592

    The FA Core Complex Contains a Homo-dimeric Catalytic …

  22. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7613635

    The DNA-damage kinase ATR activates the FANCD2-FANCI …

  23. Cell Press
    https://www.cell.com/cell-reports/fulltext/S2211-1247(23)01622-4

    The FANCI/FANCD2 complex links DNA damage response to R …

  24. Nature
    https://www.nature.com/articles/s41419-022-04952-0

    FANCD2 maintains replication fork stability during ... - Nature

  25. ResearchGate
    https://www.researchgate.net/figure/FANCI-forms-a...

    FANCI forms a monomer and binds DNA whereas FANCD2 is