fancd2 homodimer - Search
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  1. Nature
    https://www.nature.com/articles/s41594-020-0380-1

    FANCD2–FANCI is a clamp stabilized on DNA by

    • In contrast, isolated FANCD2 is a homodimer that is unable to bind DNA, suggestive of an autoinhibitory mechanism that prevents premature activation. Together, our work suggests that FA… See more

    Cloning, Expression and Purification

    cDNAs encoding full-length Gallus gallus FANCI and FANCD2 were synthesized (GeneArt) and cloned into pACEBac1. The individual genes were amplified by PCR and cloned … See more

    Nature
    In Vitro Ubiquitination and Purification of Ubiquitinated D2–I

    Based on previously described ubiquitination assays8,28, we set up a large-scale in vitro … See more

    Nature
    Electrophoretic Mobility Shift Assays

    Fluorescently labeled DNA (6FAM-labeled or Alexa647-labeled on the 3ʹ end, purchased from IDT) was prepared by incubating complementary oligonucleotides (Suppleme… See more

    Nature
    Gel Filtration Assays

    A volume of 300 µl of purified D2–I, FANCD2 and FANCI at 1 µM were sequentially run on a Superdex 200 10/300 column (GE Healthcare Life Sciences) equilibrate… See more

    Nature
    Protein Binding Assays

    We used purified StrepII-tagged FANCD2 and His-tagged FANCI to probe their interaction. In a total volume of 50 µl per reaction, FANCD2:FANCI at molar ratios 1:0, 1:0.5, 1… See more

    Nature
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    Table of Contents
    Cloning, Expression and Purification
    In Vitro Ubiquitination and Purification of Ubiquitinated D2–I
    Electrophoretic Mobility Shift Assays
    Gel Filtration Assays
    Protein Binding Assays
     

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  2. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7067600

    FANCD2–FANCI is a clamp stabilized on DNA by …

     
  3. PubMed
    https://pubmed.ncbi.nlm.nih.gov/32066963

    FANCD2-FANCI is a clamp stabilized on DNA by ... - PubMed

  4. Studies of fancd2 homodimer
    National Center for Biotechnology Information
    FANCD2–FANCI is a clamp stabilized on DNA by monoubiquitination of FANCD2 during DNA repair
    Vertebrate DNA crosslink repair excises toxic replication-blocking DNA crosslinks. Numerous factors involved in crosslink repair have been identified, and mutations in their corresponding genes cause Fanconi anemia (FA). A key step in crosslink repair
    Author: Pablo Alcón, S…
    Published: 2020/03
    DOI: 10.1038/s41594-0…
    PubMed
    FANCD2-FANCI is a clamp stabilized on DNA by monoubiquitination of FANCD2 during DNA repair
    Vertebrate DNA crosslink repair excises toxic replication-blocking DNA crosslinks. Numerous factors involved in crosslink repair have been identified, and mutations in their corresponding genes cause Fanconi anemia (FA). A key step in crosslink repair
    Author: Pablo Alcón
    Published: 2020/3
    Keywords: National In…
    National Center for Biotechnology Information
    Structural insight into FANCI–FANCD2 monoubiquitination
    The Fanconi anemia (FA) pathway coordinates a faithful repair mechanism for DNA damage that blocks DNA replication, such as interstrand cross-links. A key step in the FA pathway is the conjugation of ubiquitin on to FANCD2 and FANCI, which is facilitated
    Author: Landing Li, Wi…
    Published: 2020/10
    Publication: Essays Bi…
    ResearchGate
    FANCD2–FANCI is a clamp stabilized on DNA by monoubiquitination of FANCD2 during DNA repair
    Vertebrate DNA crosslink repair excises toxic replication-blocking DNA crosslinks. Numerous factors involved in crosslink repair have been identified, and mutations in their corresponding genes cause Fanconi anemia (FA). A key step in crosslink repair
    Published: 2020/03/01
    DOI: 10.1038/s41594-0…
  5. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7588663

    Structural insight into FANCI–FANCD2 monoubiquitination

  6. Nature
    https://www.nature.com/articles/s41586-024-07770-w

    FANCD2–FANCI surveys DNA and recognizes double- to single …

  7. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7343854

    Ubiquitilated Fanconi ID complex embraces DNA - PMC

  8. People also ask
    Does FANCD2 bind to DNA?
    In contrast, isolated FANCD2 is a homodimer that is unable to bind DNA, suggestive of an autoinhibitory mechanism that prevents premature activation. Together, our work suggests that FANCD2–FANCI is a clamp that is locked onto DNA by ubiquitin, with distinct interfaces that may recruit other DNA repair factors.

    FANCD2–FANCI is a clamp stabilized on DNA by ... - Nature

    nature.com
    Is FANCD2 a homodimer?
    Because the D2–I heterodimer is favored over the FANCD2 homodimer, it is unlikely that there would be substantial amounts of monomeric FANCI and homodimeric FANCD2 in the cell. However, it remains possible that the dynamics of D2–I association may be modulated by post-translational modifications.

    FANCD2–FANCI is a clamp stabilized on DNA by ... - Nature

    nature.com
    Does FANCD2 contain a Homo-dimeric catalytic module?
    The FA core complex contains a homo-dimeric catalytic module for the symmetric mono-ubiquitination of FANCI-FANCD2. Cell Reports 18, 611–623 (2017). Liang, C. C. et al. The FANCD2–FANCI complex is recruited to DNA interstrand crosslinks before monoubiquitination of FANCD2. Nat. Commun. 7, 12124 (2016).

    FANCD2–FANCI is a clamp stabilized on DNA by ... - Nature

    nature.com
    Can a FANCD2 homodimer be ubiquitinated?
    Here, we show that the FANCD2 homodimer adopts a closed conformation that is unable to engage DNA. It cannot be ubiquitinated because the monoubiquitinated lysines are occluded at the dimer interface.

    FANCD2–FANCI is a clamp stabilized on DNA by ... - Nature

    nature.com
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  9. Nature
    https://www.nature.com/articles/s41422-020-0345-2

    Ubiquitilated Fanconi ID complex embraces DNA | Cell Research

  10. ResearchGate
    https://www.researchgate.net/publication/…

    FANCD2–FANCI is a clamp stabilized on DNA by

    WEBMar 1, 2020 · When incubated with free FANCI, the FANCD2 homodimer exchanges to D2-I, acquiring DNA binding properties. This suggests an autoinhibitory mechanism that prevents FANCD2 activation on DNA until...

  11. bioRxiv
    https://www.biorxiv.org/content/biorxiv/early/2019/11/28/858225.full.pdf
    [PDF]

    FANCD2-FANCI is a clamp stabilized on DNA by ... - bioRxiv

  12. bioRxiv
    https://www.biorxiv.org/content/10.1101/858225v1

    FANCD2-FANCI is a clamp stabilized on DNA by ... - bioRxiv

  13. Radcliffe Department of Medicine
    https://www.rdm.ox.ac.uk/publications/1114041

    FANCD2-FANCI is a clamp stabilized on DNA by …

  14. RCSB PDB
    https://www.rcsb.org/structure/6tni

    6TNI: Structure of FANCD2 homodimer - RCSB PDB

  15. ResearchGate
    https://www.researchgate.net/figure/FANCI-forms-a...

    FANCI forms a monomer and binds DNA whereas FANCD2 is

  16. Portland Press
    https://portlandpress.com/essaysbiochem/article/64/...

    Structural insight into FANCI–FANCD2 monoubiquitination

  17. ResearchGate
    https://www.researchgate.net/figure/Model-for...

    Model for regulation of FANCD2 and FANCI in DNA crosslink repair.

  18. Nature
    https://www.nature.com/articles/s41422-020-0345-2.pdf
    [PDF]

    Ubiquitilated Fanconi ID complex embraces DNA - Nature

  19. EMBL-EBI
    https://www.ebi.ac.uk/emdb/EMD-10534

    EMDB < EMD-10534 - EMBL-EBI

  20. Europe PMC
    https://europepmc.org/article/MED/32066963

    FANCD2-FANCI is a clamp stabilized on DNA by …

  21. ScienceDirect
    https://www.sciencedirect.com/science/article/pii/S0891584913015335

    Aldehyde dehydrogenase 3A1 protects airway epithelial cells from ...

  22. ResearchGate
    https://www.researchgate.net/figure/FANCD2-dimers...

    FANCD2 dimers cannot be ubiquitinated but they exchange with …

  23. firstalert4.com
    https://www.firstalert4.com

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  24. Nature
    https://www.nature.com/articles/leu201393

    mTOR regulates DNA damage response through NF-κB …

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