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  1. Nature
    https://www.nature.com/articles/s41594-020-0380-1

    FANCD2–FANCI is a clamp stabilized on DNA by ... - Nature

    • cDNAs encoding full-length Gallus gallus FANCI and FANCD2 were synthesized (GeneArt) and cloned into pACEBac1. The individual genes were amplified by PCR and cloned into pBIG1a vector using a modi… See more

    In Vitro Ubiquitination and Purification of Ubiquitinated D2–I

    Based on previously described ubiquitination assays8,28, we set up a large-scale in vitro reconstitution of the FA core complex−mediated ubiquitination of D2–I. In a t… See more

    Nature
    Electrophoretic Mobility Shift Assays

    Fluorescently labeled DNA (6FAM-labeled or Alexa647-labeled on the 3ʹ end, purchased from … See more

    Nature
    Gel Filtration Assays

    A volume of 300 µl of purified D2–I, FANCD2 and FANCI at 1 µM were sequentially run on a Superdex 200 10/300 column (GE Healthcare Life Sciences) equilibrate… See more

    Nature
    Protein Binding Assays

    We used purified StrepII-tagged FANCD2 and His-tagged FANCI to probe their interaction. In a total volume of 50 µl per reaction, FANCD2:FANCI at molar ratios 1:0, 1:0.5, 1… See more

    Nature
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    Table of Contents
    In Vitro Ubiquitination and Purification of Ubiquitinated D2–I
    Electrophoretic Mobility Shift Assays
    Gel Filtration Assays
    Protein Binding Assays
     

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  2. Studies of fanci fancd2
     
  3. Nature
    https://www.nature.com/articles/s41586-024-07770-w

    FANCD2–FANCI surveys DNA and recognizes double- to single …

     
  4. Cell Press
    https://www.cell.com/cell-reports/fulltext/S…

    The FANCI/FANCD2 complex links DNA damage …

    WEBJan 23, 2024 · The central FA protein complex FANCI/FANCD2 (ID2) is activated by monoubiquitination and recruits DNA repair proteins for interstrand crosslink (ICL) repair and replication fork protection. Defects …

  5. Nature
    https://www.nature.com/articles/s41594-022-00820-9

    The DNA-damage kinase ATR activates the FANCD2-FANCI …

  6. PubMed
    https://pubmed.ncbi.nlm.nih.gov/32066963

    FANCD2-FANCI is a clamp stabilized on DNA by ... - PubMed

  7. Images of FANCI FANCD2

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  8. EMBO Press
    https://www.embopress.org/doi/full/10.152…

    Differential functions of FANCI and FANCD2

    WEBJun 8, 2020 · Non‐ubiquitinated/deubiquitinated FANCI or FANCD2 proteins were distinguished from respective ubiquitinated products/substrates following SDS–PAGE on Novex 4–12% Tris‐glycine …

  9. PubMed
    https://pubmed.ncbi.nlm.nih.gov/34137174

    Mechanism, specificity, and function of FANCD2-FANCI

  10. Cell Press
    https://www.cell.com/cell/pdf/S0092-8674(24)00892-4.pdf
    [PDF]

    The Fanconi anemia pathway induces chromothripsis and …

  11. PubMed
    https://pubmed.ncbi.nlm.nih.gov/39181133

    The Fanconi anemia pathway induces chromothripsis and ecDNA …

  12. Cell Press
    https://www.cell.com/molecular-cell/fulltext…

    FBXL12 degrades FANCD2 to regulate replication …

    WEBAug 16, 2023 · Fanconi anemia (FA) signaling, a key genomic maintenance pathway, is activated in response to replication stress. Here, we report that phosphorylation of the pivotal pathway protein FANCD2 by CHK1 …

  13. eLife
    https://elifesciences.org/articles/54128

    Monoubiquitination by the human Fanconi anemia …

    WEBMar 13, 2020 · FANCI:FANCD2 monoubiquitination is a critical event for replication fork stabilization by the Fanconi anemia (FA) DNA repair pathway. It has been proposed that at stalled replication forks, …

  14. Nature
    https://www.nature.com/articles/s41586-020-2110-6

    DNA clamp function of the monoubiquitinated Fanconi anaemia …

  15. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3310437

    Structure of the FANCI-FANCD2 Complex: Insights into the …

  16. Science | AAAS
    https://www.science.org/doi/10.1126/science.1205805

    Structure of the FANCI-FANCD2 Complex: Insights into the

  17. Nature
    https://www.nature.com/articles/s41594-022-00826-3

    The key to the FANCD2–FANCI lock - Nature

  18. oup.com
    https://academic.oup.com/nar/article/45/20/11837/4559113

    FANCI and FANCD2 have common as well as independent …

  19. American Society of Hematology
    https://ashpublications.org/blood/article/138...

    Fanci-FANCD2 Promotes Genome Stability and DNA Repair By …

  20. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11358013

    FANCD2–FANCI surveys DNA and recognizes double- to single …

  21. Science | AAAS
    https://www.science.org/doi/10.1126/science.1192656

    FAN1 Acts with FANCI-FANCD2 to Promote DNA Interstrand …

  22. Cell Press
    https://www.cell.com/cell-reports/fulltext/S2211-1247(18)32044-8

    Binding of FANCI-FANCD2 Complex to RNA and R-Loops

  23. Nature
    https://www.nature.com/articles/s42003-021-01647-8

    FANCD2 modulates the mitochondrial stress response to prevent …