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  2. Transport tissues on dry ice to cyrostat to prevent thawing. Transfer tissue to cryostat chamber (set at -20 to -24 °C to avoid full thawing of tissues) and let them equilibrate for 30 min. Turn the Peltier bay cooling on. If the cryostat does not have this cooling, use aerosol cooling spray to quickly freeze the OCT.
    Author: Ajay Kumar, Anthony Accorsi, Younghwa Rhee, Mahasweta Girgenrath
    Publish Year: 2015
    DOI: 10.3791/52793
    Publication: J Vis Exp. 2015; (99): 52793.
    Published: 2015

    Do's and Don'ts in the Preparation of Muscle Cryosections for ...

    www.ncbi.nlm.nih.gov/pmc/articles/PMC4542757/
    www.ncbi.nlm.nih.gov/pmc/articles/PMC4542757/
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  3. People also ask
    How do you freeze a tissue after a cryosection?
    Freezing the tissue should be a quick process using isopentane or liquid nitrogen (which is the most common) to prevent ice crystal formation, which will change morphological shape. Slide 21 Histology Hack #3: Right temperature will save a lot of trouble when cryosectioning

    Histology Hacks for Cryosectioning | Leica Biosystems

    leicabiosystems.com
    What would be the result of freezing off a melanoma?

    Dr. Yosuke Hirasawa

    Doctor of Medicine - MD · 11 years of exp

    Cryotherapy is not a common treatment for melanoma. Cryotherapy may be used for basal cell carcinoma. The basic treatment for melanoma is surgical removal of the tumor and, if necessary, lymph node dissection. If there are distant metastases, chemotherapy or radiation therapy is given.
    Q&A: Melanoma
    microsoftstart.msn.com
    Can tissue freezing and processing be used in a muscle cryosection?
    The current workflow can concurrently facilitate tissue freezing and processing for an excellent muscle cryosection, with a clearly visible cytoplasmic compartment and the tight apposition of myofibers to the surrounding tissue.

    A Multi-hole Cryovial Eliminates Freezing Artifacts when Muscle Tissues

    ncbi.nlm.nih.gov
    Can frozen sections be used for cryosection?
    Frozen sections may be used for a variety of procedures, including immunochemistry, enzymatic detection, and in situ hybridization. A protocol for cryosectioning is presented here. Related procedures for preparing tissues are described in the following CSH Protocols articles: () and ().

    Cryosectioning Tissues - CSH Protocols

    cshprotocols.cshlp.org
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  4. Leica Biosystems
    https://www.leicabiosystems.com/know…

    Cryosectioning Overview: Protocol for Sectioning …

    WEBThis novel system uses simple techniques and apparatus to accomplish face down embedding in freezing temperature steel wells. The system …

    • Estimated Reading Time: 12 mins
       
    • Feinberg School of Medicine
      https://www.feinberg.northwestern.edu/sites/mhpl/...
      [PDF]

      TISSUE FREEZING METHODS FOR CRYOSTAT …

    • The University of Alabama at Birmingham | UAB
      https://www.uab.edu/research/home/cpl-freezing...

      Freezing Tissues For Cryosectioning | Office of Research

    • uab.edu
      https://cardiology.uab.edu/research/home/cpl...

      Freezing Tissues For Cryosectioning | Office of Research

    • YouTube
      https://www.youtube.com/watch?v=x0rzrDU9h0U

      Cryosectioning of Fixed and Frozen Tissue | YouTube

    • Cold Spring Harbor Protocols
      https://cshprotocols.cshlp.org/content/2008/8/pdb.prot4991.full

      Cryosectioning Tissues | CSH Protocols

    • Leica Biosystems
      https://www.leicabiosystems.com/us/educational...

      Histology Hacks for Cryosectioning | Leica Biosystems

    • Springer
      https://link.springer.com/protocol/10.1007/978-1-0716-3585-8_13

      Cryosectioning and Immunohistochemistry Using Frozen Adult

    • Nature
      https://www.nature.com/articles/nprot.2007.365

      Cryosectioning and immunolabeling | Nature Protocols

    • Springer
      https://link.springer.com/protocol/10.1007/978-1-62703-444-9_28

      Cryosectioning | SpringerLink

    • National Center for Biotechnology Information
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10651725

      A technology platform for standardized cryoprotection and …

    • Nature
      https://www.nature.com/articles/s41598-017-06942-1

      A Cryosectioning Technique for the Observation of Intracellular ...

    • Nature
      https://www.nature.com/articles/nprot.2007.365.pdf
      [PDF]

      Cryosectioning and immunolabeling | Nature

    • Current Protocols
      https://currentprotocols.onlinelibrary.wiley.com/doi/pdf/10.1002/cpz1.342
      [PDF]

      'Cryosectioning'. In: Current Protocols

    • Wikipedia
      https://en.wikipedia.org/wiki/Frozen_section_procedure

      Frozen section procedure | Wikipedia

    • National Center for Biotechnology Information
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542757

      Do's and Don'ts in the Preparation of Muscle Cryosections for ...

    • PubMed
      https://pubmed.ncbi.nlm.nih.gov/21356883

      Cryosectioning tissues | PubMed

    • Springer
      https://link.springer.com/protocol/10.1007/978-1-62703-776-1_13

      Cryosectioning Fixed and Cryoprotected Biological Material for ...

    • Bitesize Bio
      https://bitesizebio.com/28466

      Can You Stand the Cold? Cryosectioning for Beginners | Bitesize …

    • PubMed
      https://pubmed.ncbi.nlm.nih.gov/23681639

      Cryosectioning | PubMed

    • National Center for Biotechnology Information
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5564479

      A Multi-hole Cryovial Eliminates Freezing Artifacts when Muscle …

    • PubMed
      https://pubmed.ncbi.nlm.nih.gov/19340807

      Cryosectioning | PubMed

    • Nature
      https://www.nature.com/articles/s41551-022-00952-9

      A deep-learning model for transforming the style of tissue ... | Nature

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