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  2. Freeze a fresh, unfixed tissue sample, up to 2.0 cm in diameter, in OCT in a suitable tissue mold. Freeze the OCT containing the tissue onto the specialized metal grids that fit onto the cryostat. OCT is viscous at room temperature and miscible with H2O, but freezes into a solid support at −20°C.
    cshprotocols.cshlp.org/content/2008/8/pdb.prot4991.full
    cshprotocols.cshlp.org/content/2008/8/pdb.prot4991.full
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  4. Cryosectioning Overview: Protocol for Sectioning …

    In conventional cryostats, tissue is embedded for frozen section by placing it face up on a tissue holder and covered with an embedding medium. The tissue holder or "chuck" is then set upon a freezing temperature bar.

  5. Freezing Tissues For Cryosectioning - Office of Research

  6. R&D Systems Frozen Tissue Preparation and IHC …

    This method utilizes frozen tissues that are fixed after snap-freezing and sectioning with a cryostat. Immediately snap freeze fresh tissue in isopentane mixed with dry ice, and keep at -70 °C. Do not allow frozen tissue to thaw …

  7. Frozen section procedure - Wikipedia

  8. Cryosectioning Tissues - CSH Protocols

  9. Cryostat sectioning of frozen tissues – PROMETHEUS

    Jam your frozen tissue quickly into the medium, and arrange in the best orientation for sectioning. Freeze the tissue onto the stub by plunging into liquid nitrogen or placing into the -20 ∘ C freezer. Do not freeze too cold, or the …

  10. Pathology Outlines - Frozen section procedure

    Oct 1, 2011 · Cryowells: useful in keeping all tissue on an even plane; also helpful in eliminating loss of smaller tissues that are frozen with larger ones, although recommended to not freeze different sizes together

  11. Do's and Don'ts in the Preparation of Muscle Cryosections for ...

  12. Freezing Tissues For Cryosectioning - Office of Research

  13. Frozen Section Technique III — Pathology Innovations

  14. Can You Stand the Cold? Cryosectioning for Beginners - Bitesize …

  15. How to Safely Prepare and Handle Frozen Tissue - PathSUPPLY

  16. Cryosectioning tissues - PubMed

  17. (PDF) Cryosectioning Tissues - ResearchGate

  18. Histology Hacks for Cryosectioning - Leica Biosystems

  19. Cryogenic Freezing: All you need to know - susupport.com

  20. Cryosectioning Overview: Protocol for Sectioning Frozen Tissue