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Miniscope GRIN Lens System for Calcium Imaging of Neuronal …
This article includes miniscope design, the surgical procedure for GRIN lens implantation, miniscope mounting on the head of a mouse, and data acquisition and analysis.
- Author: Lifeng Zhang, Bo Liang, Giovanni Barbera, Sarah Hawes, Sarah Hawes, Yan Zhang, Kyle Stump, Ira Baum,...
- Publish Year: 2019
See results only from pubmed.ncbi.nlm.nih.govUnit Title: Miniscope GRIN l…
Combined with GRIN lens, the miniscope imaging system allows recording of …
Stereotaxic Viral Injection an…
The preparation procedure consists of three steps, including (1) stereotaxically …
Minimally invasive microend…
We designed a thin-walled polyimide guide cannula that minimizes tissue removal …
Gradient Index Microlens Im…
Micro-optic implants, such as GRIN lenses, are well suited for combined mode, …
Time course images of cellul…
Here, we present the design and use of an implanted small-diameter, high …
A Two-Step GRIN Lens Coa…
Here, we describe a custom coating method that completely prevents the toxic side …
Unit Title: Miniscope GRIN lens system for calcium imaging of …
Oct 18, 2019 · Combined with GRIN lens, the miniscope imaging system allows recording of calcium activity from hundreds of neurons in deep brain regions for months, facilitating the …
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Stereotaxic Viral Injection and Gradient-Index Lens Implantation …
The preparation procedure consists of three steps, including (1) stereotaxically injecting the virus at the desired brain region of a mouse brain to label a specific subgroup of neurons with …
Video: Stereotaxic Viral Injection and Gradient-Index Lens ... - JoVE
Oct 8, 2021 · The postmortem assessment for GCaMP6f expression and GRIN lens implantation in the medial PFC of an experimental mouse indicated that GCaMP6f was expressed and the …
Clear optically matched panoramic access channel …
Aug 5, 2021 · Such miniature lens probes typically utilize a GRIN lens for its slim cylindrical body to form a focus deep inside brain tissue and relay the optical …
- Author: Bowen Wei, Chenmao Wang, Zongyue Cheng, Zongyue Cheng, Baoling Lai, Wen-Biao Gan, Meng Cui
- Publish Year: 2021
Minimally invasive microendoscopy system for in vivo functional …
We designed a thin-walled polyimide guide cannula that minimizes tissue removal during its implantation in mouse brain, and allows easy insertion and removal of 0.5-mm-diameter GRIN …
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Stereotaxic Viral Injection and Gradient-Index Lens ... - PubMed
Oct 8, 2021 · The preparation procedure consists of three steps, including (1) stereotaxically injecting the virus at the desired brain region of a mouse brain to label a specific subgroup of …
Gradient Index Microlens Implanted in Prefrontal Cortex of Mouse …
Jan 22, 2016 · Micro-optic implants, such as GRIN lenses, are well suited for combined mode, longitudinal imaging studies in mouse brain regions that are too deep to reach through …
Calcium imaging of adult-born neurons in freely moving mice
Mar 3, 2021 · Surgically implant a gradient-index (GRIN) lens and electroencephalogram (EEG)/electromyogram (EMG) electrodes into the brains of tamoxifen-injected mice. Note: We …
The McGill‐Mouse‐Miniscope platform: A ... - Wiley Online Library
Jul 21, 2020 · The GRadient INdex (GRIN) lens, which faithfully relays images from subcortical tissues in the brain to the CMOS sensor, further improved miniscope imaging systems to …
Two-photon imaging in freely behaving mice - Nature Methods
May 11, 2022 · The MINI2P can image through a cover glass, a gradient index (GRIN) lens or a prism, which the researchers illustrated by imaging in the visual cortex, the hippocampus or …
Aberration correction in long GRIN lens-based microendoscopes …
Oct 21, 2024 · In this study, we combined optical simulations with fabrication of aspherical polymer microlenses through three-dimensional (3D) microprinting to correct for optical …
High-Resolution Confocal Imaging Inside Living Mouse Brains
May 10, 2021 · These GRIN-lens ‘miniscopes’ monitor calcium signaling of individual neurons in deep regions of the brain. However, miniscopes sacrifice image quality, optical sectioning and …
Miniscope GRIN Lens System for Calcium Imaging of Neuronal …
Oct 1, 2018 · This article includes miniscope design, the surgical procedure for GRIN lens implantation, miniscope mounting on the head of a mouse, and data acquisition and analysis.
Peering beyond the surface with Gradient Index lenses - Biodock
Sep 7, 2022 · To implant a GRIN lens in the brain, the subject (typically a mouse) is first anesthetized, and their skull is exposed. After using the sutures of the skull to bring it to level, …
Time course images of cellular injury and recovery in murine …
Here, we present the design and use of an implanted small-diameter, high-resolution gradient index (hrGRIN) lens system for longitudinal studies that is optimized to image changes in fine …
A Two-Step GRIN Lens Coating for In Vivo Brain Imaging
Here, we describe a custom coating method that completely prevents the toxic side-effects of most commercial GRIN lenses. The coated lens we describe is non-toxic, biocompatible, and …
Decoding the hidden variabilities in mPFC descending pathways …
Mar 19, 2025 · Bottom: An example of GCaMP6m expression in the mPFC with AAVretro- GCaMP6 injection in the NAc and an image of Ca 2+ fluorescence recorded with the …
H&E stain of C57 mouse retinal tissue showing the multi layered ...
Figure 1 displays an image of an H&E stained embedded C57 mouse retinal tissue section indicating the numerous cell layers and their size relative to one another.
A Two-Step GRIN Lens Coating for In Vivo Brain Imaging
Mar 9, 2019 · Here, we describe a custom coating method that completely prevents the toxic side-effects of most commercial GRIN lenses. The coated lens we describe is non-toxic, …
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