Targeted genome editing events fall into two general classes: knock-in and knock-out. The goal of a knock-out is to disrupt DNA translation by generating a frameshift mutation. T… See more

The easiest way to make a knock-in cell line is to utilize the built-in repair pathway cells already have for repairing DNA double strand breaks – (HR). HR is the dominant homology-… See more

The basis of the HDR pathway relies on repair from a template molecule, which is usually an endogenously available sister chromatid, though exogenous DNA can be used as we… See more

Like I said earlier, HDR is not the most common editing pathway for DNA repair. To tilt the scales in your favor for an HDR edit, you can try one or more of the below suggestions.… See more