Multifunctional primasesThe AEP family of primase-polymerases has diverse features beyond making only primers. In addition to priming DNA during replication, AEP enzymes may have additional functions in the DNA replication process, su… See more
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Crystal Structure of the Human Primase - PMC
Here we report the crystal structure of the full-length human primase, revealing the precise overall organization of the enzyme, the relative positions of its functional domains, and the mode of its …
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Step 2: Priming DNA with RNA | DNA and …
Primase primes the template strands with RNA primers. In this figure, the primase enzymes placed primers soon after helicase opened up the DNA to allow for reading of the template strands. Credit: M. Sutter, 2024.
4RR2: Crystal structure of human primase - RCSB PDB
Nov 5, 2014 · Here we report the crystal structure of the full-length human primase, revealing the precise overall organization of the enzyme, the relative positions of its functional domains, and the mode of its interaction with …
Mechanism and Evolution of DNA Primases - PMC
Rather, DNA polymerases require a stable primer already bound to the template strand in order to synthesize DNA. As discussed below, the primer can be as short as one nucleotide, but more commonly is around 10 nucleotides long.
"Primase structure and function" by Mark A. Griep
Sep 16, 2005 · Primase is the ssDNA-dependent RNA polymerase that synthesizes RNA primers during DNA replication. In common with all DNA and RNA polymerases, primase has …
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Crystal structure of a DNA-dependent RNA …
We have solved the crystal structure of the catalytic primase subunit from the hyperthermophilic archaeon Pyrococcus furiosus at 2.3 Å resolution by multiwavelength anomalous dispersion methods.
Meet DNA Primase: The Initiator Of DNA Replication
Aug 5, 2019 · DNA primase is an initiator of DNA replication and must require to start replication. However, for in vitro replication or PCR, DNA primer is used instead of RNA primer because the Taq DNA polymerase used in PCR can …
(PDF) Primase structure and function - ResearchGate
Sep 1, 1995 · As a primase, it has structural and functional features for initiating chain synthesis on ssDNA. Using amino acid sequence analysis the structure of Escherichia coli primase responsible for...
DNA Primase - an overview | ScienceDirect Topics
DNA primase is a DNA-dependent RNA polymerase that synthesizes short RNA primers essential for DNA replication in eukaryotic cells. It works in coordination with DNA polymerase α to …
Structures of human primase reveal design of …
Aug 29, 2013 · We exploit the atomic model of primase to map amino acids that are important for the catalytic function of the enzyme. In addition, we describe crystallographically the interaction between primase and DNA polymerase α, …
Structure and mechanism of human PrimPol, a DNA polymerase …
Oct 21, 2016 · We present the first structure of human PrimPol in ternary complex with a DNA template-primer and an incoming deoxynucleoside triphosphate (dNTP). The ability of PrimPol …
Structure of a DNA Polymerase α-Primase Domain That Docks …
May 5, 2010 · DNA polymerase α-primase (pol-prim) plays a central role in DNA replication in higher eukaryotes, initiating synthesis on both leading and lagging strand single-stranded DNA …
Communication between DNA polymerases and Replication …
3 days ago · The crystal structure shows that Rpa2 WH binds to the PriS catalytic subunit of the DNA primase, in close proximity to the Zinc finger and AEP (archaeo-eukaryotic primase) …
Crystal Structure of the Human Primase - Journal of Biological …
Here we report the crystal structure of the full-length human primase, revealing the precise overall organization of the enzyme, the relative positions of its functional domains, and the mode of its …
Eukaryotic DNA primase - ScienceDirect
Nov 1, 2000 · Eukaryotic DNA primase initiates the synthesis of all new DNA strands by synthesizing short RNA oligomers on single-stranded DNA. Additionally, primase helps couple …
Mechanism and evolution of DNA primases - ScienceDirect
May 1, 2010 · DNA primase synthesizes short RNA primers that replicative polymerases further elongate in order to initiate the synthesis of all new DNA strands. Thus, primase owes its …
Primase | Definition, Types & Function - Lesson | Study.com
Nov 21, 2023 · Primase is a DNA-dependent enzyme with a crystal-like structure in humans. It is composed of 2 subunits where 1 is the regulatory unit and the other is the catalytic unit that …
Crystal Structure of the Human Primase - PMC - National Center …
Feb 2, 2015 · Here we report the crystal structure of the full-length human primase, revealing the precise overall organization of the enzyme, the relative positions of its functional domains, and …
Identification of a DNA primase template tracking site redefines …
Using a new screening method to trap transient macromolecular interactions, we determined the structure of the Escherichia coli DnaG primase catalytic domain bound to single-stranded …
Structure and mechanism of human PrimPol, a DNA polymerase …
We present the first structure of human PrimPol in ternary complex with a DNA template-primer and an incoming deoxynucleoside triphosphate (dNTP). The ability of PrimPol to function as a …
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