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Large-scale production of lentiviral vector in a closed system …
To generate a large scale, partially closed system method for the manufacturing of clinical grade lentiviral vector suitable for the generation of induced pluripotent stem cells (iPSCs), we …
Highly Efficient Large-Scale Lentiviral Vector Concentration by …
Large volumes of LV supernatant can be produced easily through the use of multi-layer flasks, each having 1720 cm 2 surface area and producing ~560 mL of supernatant per flask. …
Optimization of Culture Media and Feeding Strategy for High Titer ...
A maximum viable cell density up to 16 × 10 6 cells/mL was achieved in shake flask fed-batch cultures using the selected media and commercial or in-house developed feeds. The …
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