In a first experiment we used the first SLM (see Fig. 1) to generate a simple holographic shape (a 15-µm diameter round spot) that was focused onto the diffraction grating for … See more

As described in ref.27, the MTF-MS method relies on a mixed phase/amplitude shaping approach, capable of simultaneously producing different speckle-free shapes, with an ove… See more

We next used the GRIN lens based ME to perform concurrent holographic stimulation of single or multiple cells and optical readout of population activity in vivo in mice. As pr… See more

Through the GRIN lens, we were able to resolve on average 34 ± 3 individual cortical neurons in a FOV of 150 × 150 µm2 (mean ± s.e.m., 9 FOVs) at different depths (between 1… See more