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Influenza viruses rely on target cells to take up particles into their endocytic machinery for initiation of viral replication. This has been carefully characterized mostly in epithelial cell lines …

In this chapter, three protocols (negative staining, chemical processing, and high-pressure freezing-freeze substitution) routinely used in the Electron Microscopy Facility at the Instituto …

Feb 1, 2025 · In this chapter, we will present three different techniques for transmission electron microscopy (TEM) that we routinely use in the Electron Microscopy Facility at the Instituto …

5 days ago · Enveloped viruses such as influenza A virus (IAV) often produce a mixture of virion shapes, ranging from 100 nm spheres to micron-long filaments. ... Nakai, M. & Imagawa, D. T. …

The genome of influenza A viruses consists of eight single-stranded RNA segments, and the viral particle has two major glycoproteins on its surface: hemagglutinin and neuraminidase. This …

Yet we have a limited understanding of the viral replication cycle at molecular resolution. Electron microscopy of resin-embedded samples has traditionally been employed to investigate viral …

6 days ago · Influenza A virus particles strategically adapt their shape – to become either spheres or larger filaments – to favor their ability to infect cells depending on environmental conditions, …

Dec 11, 2024 · A transmission electron microscope (TEM) image shows a rod-shaped H5N1 (bird flu) virus, which is a subtype of the influenza A virus. In the background, a second TEM image …

Thanks to some advanced electron microscopy from UW School of Pharmacy’s Associate Professor of Medicinal Chemistry Kelly Lee’s laboratory, we are starting to understand how the …

Nov 21, 2019 · Electron microscopy is one of the powerful tool for direct visualization of influenza viruses at nanometer resolution. In research and diagnosis, transmission electron microscopy …

Aug 20, 1985 · An electron microscopical study was made of the influenza virus, type B/Hong Kong, in the unstained, frozen, hydrated state after quench-freezing in cooled liquid ethane. …

A rapidly frozen vitrified aqueous suspension of influenza A virus was observed by high resolution electron cryomicroscopy. The influenza particles were grouped into small (diameter < 150 nm) …

5 days ago · Colorized transmission electron micrograph of influenza A/H3N2 virus particles, isolated from a patient sample and then propagated in cell culture. Influenza A virus particles …

Aug 28, 2018 · The light microscopy stage enables visualization of fluorescently tagged viral particles and allows study of dynamic or rare intracellular trafficking events, while electron …

We combine fluorescence microscopy of virus-infected human lung carcinoma A549 cells with high-pressure freezing (HPF) and in-resin fluorescence CLEM and the Tokuyasu CLEM …

Sep 1, 2015 · In this review, we survey EM-based contributions towards the characterization of influenza virus-mediated membrane fusion and anticipate the potential for future …

Sep 1, 2015 · Briefly, cryo-electron microscopy (cryo-EM) and cryo-electron-tomography (cryo-ET) have shown the structure of influenza virions at neutral pH in close-to-native conditions; thin …

Feb 6, 2025 · Here, we use negative stain electron microscopy polyclonal epitope mapping (nsEMPEM) to structurally characterize the humoral IgG antibody responses to hemagglutinin …

Recently improved methods for visualization of thin tissue sections by electron microscopy have been applied to the study of early changes in the bronchial epithelium of mice infected by …

9 hours ago · Intranasal vaccination is a desired route for protection against influenza viruses by mucosal and systemic immunity. However, the nasal mucosa impedes the intranasal delivery …

Aug 20, 1985 · An electron microscopical study was made of the influenza virus, type B/Hong Kong, in the unstained, frozen, hydrated state after quench-freezing in cooled liquid ethane. …

Equine influenza (EI) is a highly contagious acute respiratory disease of wild and domesticated horses, donkeys, mules, and other Equidae. EI is caused by the Equine Influenza virus (EIV), …