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OverviewSYBR Gold is an asymmetrical cyanine dye. It can be used as a stain for double-stranded DNA, single-stranded DNA, and RNA. SYBR Gold is the most sensitive fluorescent stain of the SYBR family of dyes for the dete… See more
Watch full videoFluorescence propertiesSYBR Gold has two fluorescence excitation maxima when bound to DNA, one centered at ~300 nm and one at ~495 nm, and a single emission maximum at ~537 nm. SYBR Gold exhibits improved DNA cloning efficiency c… See more
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SYBR™ Gold Nucleic Acid Gel Stain (10,000X …
SYBR Gold nucleic acid gel stain is the most sensitive gel stain available for detecting dsDNA, ssDNA and RNA in native, glyoxal, formaldehyde or urea gels. Use with UV transilluminators, the Dark Reader, or laser scanners.
Molecular Probes SYBR® Gold nucleic acid gel stain is the most sensitive fluorescent stain available for detecting double- or single-stranded DNA or RNA in electrophoretic gels, using …
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SYBR Gold (Invitrogen), a fluorescent dye, detects double- and single-stranded DNA and RNA and is more sensitive than SYBR Green I for staining these nucleic acids on gels (Tuma et al. …
- File Size: 326KB
- Author: Akira Shibata, Yoichi Goto, Hiroaki Saito, Tomohiko Kikuchi, Tatuki Toda, Satoru Taguchi
- Page Count: 9
- Publish Year: 2006
SYBR Gold dye enables preferential labelling of …
Sep 18, 2018 · Organic DNA-binding dyes are free of these drawbacks, but they lack specificity to mitochondrial DNA. Here, considering physico-chemical …
- Author: Visnja Jevtic, Petra Kindle, Sergiy V. Avilov
- Estimated Reading Time: 9 mins
- Publish Year: 2018
Molecular structure, DNA binding mode, photophysical properties …
To determine the binding mode of SYBR Gold to DNA and to investigate the fluorescence response under varying DNA and SYBR Gold concentrations, we combined single-molecule …
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However using the Thermo Scientific NanoDropTM 3300 Fluorospectrometer, Sybr® Gold can be used to quantitate purified dsDNA, RNA, and ssDNA quickly and accurately. With few viable …
Oxazole Gold (SYBR® Gold), 10,000X in DMSO
Oxazole Gold, also known as SYBR® Gold, is the most sensitive fluorescent gel stain available for DNA and RNA. Biotium's 1 kb DNA ladder or single-stranded RNA ladder (NEB) were run on a 1% agarose TBE gel at 0.1 ug DNA (lane 1), …
Comparison of SYBR Green I and SYBR Gold Stains for …
Thus we compared bacterial and viral counts using SYBR Gold and SYBR Green I stains. Variables for both bacterial and viral counts included season and ocean depths of sample collection and the period of sustained excitation under epifluorescence microscopy.
SYBR Gold dye enables preferential labelling of mitochondrial
Sep 18, 2018 · Here, considering physico-chemical properties of such dyes, we achieved preferential live-cell labelling of mitochondrial nucleoids by a nucleic acid staining dye SYBR …
Application of Digital Image Analysis and Flow Cytometry To …
A novel nucleic acid stain, SYBR Gold, was used to stain marine viral particles in various types of samples. Viral particles stained with SYBR Gold yielded bright and stable fluorescent signals …
Preparation of SYBR Gold Solution Mix 5 µL SYBR Gold with 50 mL buffer to create new stock solution for staining Use the same buffer (TBE or TAE) which was used for the electrophoresis
Comparison of SYBR Green I and SYBR Gold Stains for …
Aug 19, 2006 · SYBR Gold staining is used for enumerating bacteria and viruses in aquatic samples. However, its suitability for epifluorescence microscopy has not been sufficiently …
Effect of incubation duration and concentration on SYBR Gold ...
SYBR Gold dye enables preferential labelling of mitochondrial nucleoids and their time-lapse imaging by structured illumination microscopy Article Full-text available
Molecular structure, DNA binding mode, photophysical ... - PubMed
May 21, 2021 · The MT assay reveals systematic lengthening and unwinding of DNA by 19.1° ± 0.7° per molecule upon binding, consistent with intercalation, similar to the related dye SYBR Green I. We complement the MT data with spectroscopic characterization of SYBR Gold.
Intercalative DNA binding governs fluorescence enhancement of …
May 26, 2020 · SYBR Gold is a commonly used and particularly bright fluorescent DNA stain, however, its binding mode to DNA remains controversial. Here, we quantitate SYBR Gold …
SYBR Gold dye enables preferential labelling of - ProQuest
Our study of SYBR Gold emphasizes that other organic dyes widely used in vitro may be useful for fluorescence microscopy or other cell-based technique like flow cytometry. In general, …
Comparison of SYBR Green I and SYBR Gold stains for …
SYBR Gold staining is used for enumerating bacteria and viruses in aquatic samples. However, its suitability for epifluorescence microscopy has not been sufficiently investigated. Thus we …
SYBR Gold dye enables preferential labelling of mitochondrial …
Here, considering physico-chemical properties of such dyes, we achieved preferential live-cell labelling of mitochondrial nucleoids by a nucleic acid staining dye SYBR Gold. It enabled time …
Inter Research » AME » v43 » n3 » p223-231
ABSTRACT: SYBR Gold staining is used for enumerating bacteria and viruses in aquatic samples. However, its suitability for epifluorescence microscopy has not been sufficiently investigated. Thus we compared bacterial and viral counts using SYBR Gold and SYBR Green I …
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