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Fluorescent dyeSYBR Green is a fluorescent dye used in molecular biology to make DNA visible under certain light conditions123. It binds to the DNA double helix, illuminating genetic material for researchers to observe and analyze1. SYBR Green is used in gene expression studies, diagnostics, and forensic analysis1. It is a dsDNA-binding dye that intercalates nonspecifically into dsDNA, allowing measurement of the amount of PCR product3.Learn more:✕This summary was generated using AI based on multiple online sources. To view the original source information, use the "Learn more" links.SYBR Green® is a powerful fluorescent dye used in molecular biology to make DNA visible under certain light conditions. It binds to the DNA double helix, illuminating genetic material for researchers to observe and analyze. This tool is pivotal in gene expression studies, diagnostics, and forensic analysis.www.allthescience.org/what-is-sybr-green.htmThe Sybr®Green I dye is a fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA). Used in con- junction with the micro-volume capability of the Thermo Sci- entific NanoDrop™3300 Fluorospectrometer, the Sybr® Green I assay provides a highly sensitive means of dsDNA quantitation with minimal consumption of sample.assets.thermofisher.com/TFS-Assets/CAD/manual…Green is a dsDNA-binding dye that intercalates nonspecifically into dsDNA, allowing measurement of the amount of PCR product. SYBR green molecules incorporated into DNA increases. Since the increase in fluorescence is proportional to the amount of product accumulated, SYBR Green qPCR can be used for relative DNA quantification.www.bio-rad.com/en-us/feature/sybr-green-for-qpcr…SYBR™ Green I Nucleic Acid Gel Stain, 10,000X concentrate in …
SYBR™ Green I Nucleic Acid Gel Stain is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose and polyacrylamide gels. Because SYBR™ Green I has greater sensitivity for dsDNA, it is especially useful for assays where the presence of …
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SYBR Green I - Wikipedia
SYBR Green I (SG) is an asymmetrical cyanine dye used as a nucleic acid stain in molecular biology. The SYBR family of dyes is produced by Molecular Probes Inc., now owned by Thermo Fisher Scientific. SYBR Green I binds to DNA. The resulting DNA-dye-complex best absorbs 497 nanometer blue light (λmax = 497 nm) and emits green light (λmax = 520 nm). The stain preferentially binds to d…
Wikipedia · Text under CC-BY-SA license- Estimated Reading Time: 3 mins
- Studies of SYBR Green DNA
SYBR Green I: Fluorescence Properties and Interaction with DNA …
See more on link.springer.comThe dependence of SG fluorescence upon titration into dsDNA was measured at two ionic strengths (Fig. 4). The fluorescence of SG starts to increase at the very beginning of DNA titration, demonstrating progressive binding of the dye to dsDNA. The additional increase of SG/DNA ratio leads to a decline of the slope and a fluo…- Author: A. I. Dragan, R. Pavlovic, J. B. McGivney, J. R. Casas-Finet, E. S. Bishop, R. J. Strouse, M. A. Sch...
- Estimated Reading Time: 11 mins
- Publish Year: 2012
- Published: Apr 26, 2012
SYBR Green I: fluorescence properties and interaction with DNA
In this study, we have investigated the fluorescence properties of SYBR Green I (SG) dye and its interaction with double-stranded DNA (dsDNA). SG/dsDNA complexes were studied using …
SYBR Green I - an overview | ScienceDirect Topics
SYBR Green I is a fluorescent dye that preferentially binds double-stranded DNA (dsDNA) in a sequence-independent manner. When SYBR Green I dye binds to dsDNA, the fluorescence …
SYBR Green I: Fluorescence properties and …
Apr 26, 2012 · In this study, we have investigated the fluorescence properties of SYBR Green I (SG) dye and its interaction with double-stranded DNA (dsDNA). SG/dsDNA complexes were studied using various...
Investigations on DNA intercalation and surface binding by SYBR …
The detection of double-stranded (ds) DNA by SYBR Green I (SG) is important in many molecular biology methods including gel electrophoresis, dsDNA quantification in solution and real-time …
What is SYBR Green®? (with pictures) - AllTheScience
May 21, 2024 · SYBR Green® allows the researcher to visualize DNA in an acrylamide gel by shining light of the appropriate wavelength on it. Other uses of SYBR Green® include detection of nucleic acids in solution, in biochip …
Demonstration of preferential binding of SYBR Green I to specific …
Nov 11, 2003 · Various double-stranded DNA (dsDNA)-specific intercalating dyes have been evaluated (8,9), but the most commonly used is SYBR Green I (SG) in both commercial and in …
The Sybr® Green I dye is a fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA). Used in con-junction with the micro-volume capability of the Thermo Sci-entific …
SYBR® Green I Nucleic Acid Stain - Lonza
SYBR ® Green II detects 100 pg ssDNA and 2 ng RNA in agarose and polyacrylamide gels. SYBR ® Green II is optimal for RNA gel electrophoresis and SSCP analysis. Both stains are supplied as a 10,000X concentrated solution …
Comparison of multiple DNA dyes for real-time PCR: effects of …
Many applications utilize SYBR Green I dye to follow the accumulation of amplicons in real time. SYBR Green I has, however, a number of limitations that include the inhibition of PCR, …
PCR with the fluorogenic DNA stain SYBR® Green I
Jan 1, 2001 · SYBR® Green I is a sensitive stain for DNA in agarose gels (Ref. [1]). Its enormous increase in fluorescence upon binding to double-stranded DNA has led to its use in monitoring …
SYTO dyes and EvaGreen outperform SYBR Green in real-time …
This paper confirms and extends previous findings that among commercially available DNA dyes, EvaGreen and certain SYTO dyes are the most desirable alternatives to the commonly used …
Investigations on DNA intercalation and surface binding by SYBR …
Jul 12, 2004 · Biophysical studies at defined dye/base pair ratios (dbprs) were used to determine the structure-property relationships that affect methods applying SG. These studies revealed …
SYBR Green I - an overview | ScienceDirect Topics
SYBR Green I is a dye that binds to double-stranded DNA during the PCR process and increases its fluorescence, allowing for the quantification of the amount of amplicon produced. However, …
How does qPCR work: SYBR Green vs TaqMan | INTEGRA
May 11, 2022 · qPCR relies on fluorescence from intercalating dyes or hydrolysis probes to measure DNA amplification after each thermal cycle. The most common dye-based method is …
Universal SYBR Green qPCR Protocol - MilliporeSigma
Learn how to perform quantitative PCR with SYBR Green I dye, a fluorescent reporter that measures DNA generation. Find out the assay considerations, methods, equipment, and …
SYBR ® Green Based Quantitative PCR - MilliporeSigma
SYBR Green for qPCR. SYBR ® Green I is a commonly used fluorescent dye that binds double-stranded DNA molecules by intercalating between the DNA bases. It is used in quantitative …
Why does SYBR green emit more signal when bound to DNA?
Jun 12, 2018 · SYBR Green I (SG) is an asymmetrical cyanine dye used as a nucleic acid stain in molecular biology. This molecule is commonly used in qPCR to meassure the amount of …
PowerUp SYBR Green Master Mix | Thermo Fisher Scientific - IN
PowerUp SYBR Green Master Mix contains proprietary Dual-Lock Taq DNA Polymerase enzyme, which uses a combination of two hot-start mechanisms to control its activity. Providing tight …
qPCR Gene Expression Protocol Using SYBR Green
Measuring a target quantity relative to one or more stable reference genes using SYBR Green I dye detection is a common application of qPCR. Below is a standard protocol that can be …
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