Sample Codes for Sul Primer

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ScienceDirect
https://www.sciencedirect.com/science/article/pii/S0048969708006670
Detection of the sul1, sul2, and sul3 genes in ... - ScienceDirect
To assess the presence and distribution of the sul genes (sul1, sul2, and sul3) and plasmids in human-mediated environments of north Vietnam, we examined a total of 127 sulfonamide-resistant (SR) bacterial i… See more
1. Introduction
Sulfonamides, synthetic antibiotics, have been widely used to treat bacterial and protozoan … See more
ScienceDirect
2. Materials and methods
2.1. Sample collection and isolation of sulfonamide-resistant (SR) bacteria
Water samples from nine sites were taken, including three fish ponds in three different VAC swine … See more
ScienceDirect
3. Results
3.1. Distribution of the sul genes
The number and percentage of isolates carrying the sul genes are summarized in Table 2. The sul1, sul2, and sul3 genes were detect… See more
ScienceDirect
4. Discussion
The distribution and spread of drug resistance genes in aquatic environments of Vietnam are of interest and are important from the viewpoint of gene transfer and public healt… See more
ScienceDirect
Acknowledgements
We thank K. Smalla, Federal Biological Research Centre for Agriculture and Forestry (BBA), Braunschweig, Germany, for supplying positive controls. We thank also P. A. … See more
ScienceDirect
How do you design a PCR primer?
The first step of designing the primers is to question whether they will be used for the conventional Polymerase Chain Reaction (PCR) or Quantitative PCR (qPCR). Real-time PCR (not to be confused with reverse-transcriptase PCR typically annotated as rt-PCR). As this is not a guide on the different types of PCRs, I will be brief.
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Table of Contents
1. Introduction
2. Materials and methods
3. Results
4. Discussion
Acknowledgements
How do you design a PCR primer?

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Studies of Sample Codes for Sul Primer
ScienceDirect
Multiplex real-time PCR assays to detect Stenotrophomonas maltophilia carrying sul1, sul2, and sul3 genes
Nosocomial infections caused by Stenotrophomonas maltophilia resistant to SXT are increasingly reported worldwide. In this study, a novel melting-curve based multiplex real-time PCR assay for the simultaneous detection of the ssrA and sul1, sul2 and sul3 genes was first established. The assays were ...
Published: 2019/01/01
Cited By: 0
Keywords: Multiplex R…
ScienceDirect
Detection of sul1 and sul2 genes in sulfonamide-resistant bacteria (SRB) from sewage, aquaculture sources, animal wastes and hospital wastewater in South-West Nigeria
Background The indiscriminate use of antibiotics has placed a lot of selective pressure on bacteria from several environments. This study aimed at detecting the occurrence of sulfonamide resistance genes and antibiogram of sulfonamide-resistant bacteria (SRB) isolated from sewage, aquaculture sourc...
Published: 2020/09/01
Cited By: 0
Keywords: SRB sulfon…
National Center for Biotechnology Information
Diverse Mobile Genetic Elements and Conjugal Transferability of Sulfonamide Resistance Genes (sul1, sul2, and sul3) in Escherichia coli Isolates From Penaeus vannamei and Pork From Large Markets in Zhejiang, China
High prevalence rates of sulfonamide resistance genes sul1, sul2, and sul3 have been observed in Gram-negative bacteria isolated from humans, domestic animals, and aquaculture species worldwide. We investigated the distribution characteristics, location, conjugative transferability, and genetic envi...
Author: Han Jiang, Hu…
Published: 2019
Publication: Front Micr…
National Center for Biotechnology Information
High abundances of class 1 integrase and sulfonamide resistance genes, and characterisation of class 1 integron gene cassettes in four urban wetlands in Nigeria
There is little information about environmental contamination with antibiotic resistance genes (ARG) in Sub-Saharan Africa, home to about 1 billion people. In this study we measured the abundance of three genes (sul1, sul2, and intI1) used as indicators of environmental contamination with ARGs in th...
Author: Olawale Olufe…
Published: 2018
Publication: PLoS On…
National Center for Biotechnology Information
Occurrence of Transferable Integrons and sul and dfr Genes Among Sulfonamide-and/or Trimethoprim-Resistant Bacteria Isolated From Chilean Salmonid Farms
Salmon farming industry in Chile currently uses a significant quantity of antimicrobials to control bacterial pathologies. The main aims of this study were to investigate the presence of transferable sulfonamide- and trimethoprim-resistance genes, sul and dfr, and their association with integrons am...
Author: Mariana Domí…
Published: 2019
Publication: Front Micr…
ResearchGate
Molecular Study on Distrbution of Sul-1 and Sul-2 genes among Salmonella enterica causing Enteric Fever
This study comprised 200 blood samples taken from patients visited some of Al-Najaf province. The most prevalent species was Salmonella Typhi, where 20 isolates had been identified. Serological typing was done by using both poly and monovalent antisera, which revealed that all isolated Salmonella wa...
Published: 2018/03/28
Bioinformatics.org
https://www.bioinformatics.org/sms2/primer_map.html
Primer Map - Bioinformatics
Use this program to produce a useful reference figure, particularly when you have designed a large number of primers for a particular template. Primer Map supports the entire IUPAC …
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Reference
lslabs.com
https://lslabs.com/resources/universal-primer-list
Universal Primer List - lslabs.com
Universal primers are PCR/sequencing primers that bind to a sequence found in many plasmid cloning vectors, most of which are derived from pUC vectors (which in turn come from pBR322).
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Universal Studios
Plasmid
Medium
https://medium.com/@zweyehtut/a-definitive...
A Definitive, Practical Guide to Designing Primers for PCR & qPCR.
Jan 5, 2022 · Primers were designed to amplify 100 bp product from gap gene and 450 bp product for beta gene, allowing easy differentiation. From this figure, we can conclude that all 3 …
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Design
Gene
Twist Bioscience
https://www.twistbioscience.com/resources/protocol...
Unique Dual Index Sequences; Reference Spreadsheets and …
This reference document provides the nucleotide sequences that comprise the Unique Dual Index (UDI) Primers of the Twist Universal Adapter System for use with Illumina sequencing …
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Dual
Unique
ScienceDirect
https://www.sciencedirect.com/science/article/pii/S0167701218304986
Multiplex real-time PCR assays to detect - ScienceDirect
Jan 1, 2019 · To confirm the specificities of the primers, singleplex real-time PCR assays were performed by using a DNA mixture from other species of bacteria that do not carry sul genes. …
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Sul Genes
Publish Year:2019
Si Li, Yuan Peng, Yongyu Rui
ResearchGate
https://www.researchgate.net/figure/Primers-Used...
Primers Used for the Identification of Sulfonamide
The literature review searched the Virtual Health Library for articles with specific data in the studied samples: the resistance genes found, the primers used in PCR, and the thermocycler...
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Resistance
Sulfonamide
ScienceDirect
https://www.sciencedirect.com/science/article/pii/S2452014420301564
Detection of sul1 and sul2 genes in sulfonamide ... - ScienceDirect
Sep 1, 2020 · This study aimed at detecting the occurrence of sulfonamide resistance genes and antibiogram of sulfonamide-resistant bacteria (SRB) isolated from sewage, aquaculture …
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Amr Resistance Gene Sul1 Sul2
Sulfate Reducing Bacteria Srb
Sul Resistance Gene
ResearchGate
https://www.researchgate.net/figure/Primers-used...
| Primers used for the specific amplification of the sul1 and sul2 ...
Objective: Our study aimed to identify the optimal antibiotic combinations that could both show high antibacterial activity against sul-carrying S. maltophilia and the ability to prevent the ...
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Amplification
Sul1
Academia.edu
https://www.academia.edu/22846436/Detection_of_the...
(PDF) Detection of the sul1, sul2, and sul3 genes in sulfonamide ...
The sul2 gene encodes sulphonamide resistance (SulR) and is commonly found in Escherichia coli from different hosts.
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Sul1
Pham Viet
Springer
https://link.springer.com/chapter/10.1007/978-3-031-45293-2_5
Primer Design - SpringerLink
Nov 28, 2023 · The criteria for selection of oligonucleotides including lengths of PCR primers and products, lengths of oligonucleotide hybridization probes, and the principles for sequence …
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Amplification
Springer Science+Business Media
Biology LibreTexts
https://bio.libretexts.org/Learning_Objects...
1.3: Designing Primers for Site-Directed Mutagenesis
Mar 19, 2021 · During the next two labs you will learn the basics of site-directed mutagenesis: you will design primers for the mutants you designed earlier and perform PCR amplification to …
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- Sul Primer
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Site-directed mutagenesis
Inquiry-based learning
National Center for Biotechnology Information
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6690019
Diverse Mobile Genetic Elements and Conjugal Transferability of ...
Aug 2, 2019 · Polymerase chain reaction (PCR) was used to detect the presence of sul genes in all sulfisoxazole-resistant E. coli isolates. The boiling method described in our previous studies …
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Sul Genes
Publish Year:2019
Published:2019
10.3389/fmicb.2019.01787
National Center for Biotechnology Information
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6264143
High abundances of class 1 integrase and sulfonamide resistance …
Nov 29, 2018 · The copy number of the 16S rRNA gene in the samples was determined by using Primers 519F and 909R [ 36] as a measure of total bacterial abundance. Standards were PCR …
Tags:
Sul Genes
Sulfonamide Resistance
Publish Year:2018
Takara Bio
https://www.takarabio.com/about/bioview-blog/tips...
Insertions, deletions, and substitutions, oh my!—designing …
Jul 27, 2021 · Our online primer design tool lets you design primers for your site-directed mutagenesis experiment simply by inputting the DNA sequences of your vector and insert(s). It …
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- Sul Primer
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Site-directed mutagenesis
DNA
National Center for Biotechnology Information
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6474311
Occurrence of Transferable Integrons and sul and dfr Genes …
Apr 12, 2019 · Strains were studied for their carriage of these genes by polymerase chain reaction, using specific primers, and 28 strains (30.8%) were found to carry at least one type of …
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Sul Genes
Publish Year:2019
Published:2019
Sul 1 Gene
nih.gov
https://pmc.ncbi.nlm.nih.gov/articles/PMC8615168
Presence of Tetracycline and Sulfonamide Resistance Genes in …
The primer sequences used to amplify tetracycline and sulfonamide resistance genes in the studies were a vital inclusion criterion, as designing appropriate primers is essential to a …
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Sulfonamide Resistance
Primer
ResearchGate
https://www.researchgate.net/figure/Primers-and...
Primers and conditions used to amplify 16S rRNA genes, sul …
... DNA was used as template. The PCR primers and conditions for amplification of sul genes and are listed in Table 1. All PCR experiments included positive controls (genomic DNA carrying sul...
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Sul Genes
Amplification
ResearchGate
https://www.researchgate.net/publication/324064295...
Molecular Study on Distrbution of Sul-1 and Sul-2 genes among ...
March 2018. Authors: Abdullah K Hindi. Ebtehal Edrees Shubbar. University Of Kufa. Salman A Addos. Assis Teacher. Citations (3) References (16) Abstract. This study comprised 200 blood …
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Fever
Salmonella enterica
Nature
https://www.nature.com/articles/s41467-023-39778-7
Molecular mechanism of plasmid-borne resistance to sulfonamide …
Jul 7, 2023 · Resistance to sulfa drugs is mediated either by mutations in folP or acquisition of sul genes, which code for sulfa-insensitive, divergent DHPS enzymes.
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Sul Genes
Sulfonamide Resistance
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Detection of the sul1, sul2, and sul3 genes in ... - ScienceDirect

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Primer Map - Bioinformatics

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Universal Primer List - lslabs.com

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A Definitive, Practical Guide to Designing Primers for PCR & qPCR.

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Unique Dual Index Sequences; Reference Spreadsheets and …

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Multiplex real-time PCR assays to detect - ScienceDirect

Primers Used for the Identification of Sulfonamide

Detection of sul1 and sul2 genes in sulfonamide ... - ScienceDirect

| Primers used for the specific amplification of the sul1 and sul2 ...

(PDF) Detection of the sul1, sul2, and sul3 genes in sulfonamide ...

Primer Design - SpringerLink

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Must include:

1.3: Designing Primers for Site-Directed Mutagenesis

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Must include:

Diverse Mobile Genetic Elements and Conjugal Transferability of ...

High abundances of class 1 integrase and sulfonamide resistance …

Insertions, deletions, and substitutions, oh my!—designing …

Missing:

Must include:

Occurrence of Transferable Integrons and sul and dfr Genes …

Presence of Tetracycline and Sulfonamide Resistance Genes in …

Primers and conditions used to amplify 16S rRNA genes, sul …

Molecular Study on Distrbution of Sul-1 and Sul-2 genes among ...

Molecular mechanism of plasmid-borne resistance to sulfonamide …

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Guidelines for Primer Design

Primer Design Guide for PCR :: Learn Designing Primers for

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