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A monolithic HPLC column, or monolithic column, is a column used in high-performance liquid chromatography (HPLC). The internal structure of the monolithic column is created in such a way that many channels form inside the column.

With flow-independent performance, low back-pressure, and versatility our monolithic columns facilitate faster purification of your viruses or nucleic acids. CIM ® monoliths are available in a variety of chemistries and sizes ranging from screening to commercial processing for higher yields and improved speed.

Aug 15, 2022 · Nowadays, monolithic columns are graining more popularity in the field of separation media for liquid chromatography. The monolith columns possess great potential as compared to the...

A monolithic column or single-piece column is a large column of which the shaft is made from a single piece of stone instead of in vertical sections. Smaller columns are very often made from single pieces of stone, but are less often described as monolithic, as the term is normally reserved for less common, larger columns made in this way.

Oct 19, 2007 · Monolithic columns have considerable advantages, which makes them most useful in many applications of liquid chromatography. They are extremely permeable and offer a high efficiency that decreases slowly with increasing flow velocity. 1. Introduction. Chromatography is a separation method that uses a fluid phase percolating through a porous bed.

Silica-based monolithic columns for preparative and semi-preparative purposes with dimensions 100 × 25 mm and 100 × 10 mm, respectively, are offered by Merck (Darmstadt, Germany). There are several examples of the use of this type of column in …

Monolithic silica columns and their use in high peak-capacity HPLC separations are reviewed. Monolithic silica columns can potentially provide higher overall performance than particle-packed columns based on the variable external porosity and …

Jan 10, 2019 · Monolithic columns provide alternatives to packed columns with potentials to improve protein separations through a combination of increased mass permeability, enhanced tolerance of sample “dirtiness”, and possibly improved resolution [1].

Dec 4, 2013 · Monolith HPLC columns have extended useful life-cycle in comparison with particulate columns due to increased shock resistance, increased temperature tolerance and ability to withstand wider pH range media.

A monolithic column is a type of column used in chromatography that is made up of a single continuous structure without any voids. This design allows for high capacity, fast separation, and high resolution.