whole mount tissue staining - Search
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  2. Whole mount staining is the staining of small pieces of tissue – usually embryos – without sectioning. Whole mount staining is very similar to immunocytochemistry (ICC) or staining of cryosections.

    IHC staining protocol for whole mount samples | abcam

    www.abcam.com/en-us/technical-resources/protocols/wholemount-staining-for-ihc
    www.abcam.com/en-us/technical-resources/protocols/wholemount-staining-for-ihc
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  3. People also ask
    What is whole-mount staining?
    Whole-mount staining preserves the three-dimensional spatial information in biological samples allowing a comprehensive interpretation of expression domains. Here we describe the samp … Immunofluorescence staining enables the visualization of protein expression at a cellular or even sub-nuclear level.

    Whole-Mount Immunofluorescence Staining of Early Mouse Embryos

    pubmed.ncbi.nlm.nih.gov
    Search for: What is whole-mount staining?
    Why do I have granules under my skin with Mast Cell?
    Mast cells are resident inflammatory cells found in the dermal layer of the skin. In uninjured tissue, mature mast cells contain abundant intracellular granules (black and gray circles), which are used to store a variety of preformed mediators. During an injury, these mast cells get activated and undergo degranulation.

    Q&A: Mast Cell

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    How can whole-mount staining improve adipose tissue anatomy?
    To overcome this limitation, whole-mount staining can be further combined with tissue clearing techniques to remove the opaqueness of tissue and allow for complete visualization of entire adipose tissue anatomy using light-sheet fluorescent microscopy.

    Visualization of 3D White Adipose Tissue Structure Using Whole-moun…

    pubmed.ncbi.nlm.nih.gov
    Search for: How can whole-mount staining improve adipose tissue anatomy?
    Can whole-mount staining be used to determine cell fate?
    In addition, whole-mount staining can be combined with lineage tracing methods to determine cell fate decisions. However, this technique has some limitations to providing accurate information regarding deeper parts of adipose tissue.

    Visualization of 3D White Adipose Tissue Structure Using Whole-moun…

    pubmed.ncbi.nlm.nih.gov
    Search for: Can whole-mount staining be used to determine cell fate?
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  4. Abcam
    https://www.abcam.com/.../wholemount-staining-for-ihc

    IHC staining protocol for whole mount samples | abcam

    • Whole mount

      The advantage of using fluorescence to stain whole mount se…

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  5. Nature
    https://www.nature.com/articles/s41467-0…

    Versatile whole-organ/body staining and imaging …

    WEBApr 27, 2020 · By using CUBIC-HistoVIsion for 3D staining, tissue clearing, and volumetric imaging, we successfully stained and imaged …

    • Author: Etsuo A. Susaki, Chika Shimizu, Akihiro Kuno, Akihiro Kuno, Kazuki Tainaka, Xiang Li, Kengo Nishi, K...
    • Publish Year: 2020
  6. Studies of whole mount tissue staining
    National Center for Biotechnology Information
    Whole-Mount Skeletal Staining - PMC - National Center for Biotechnology ...
    The first step in almost every investigation of skeletal phenotypes is analysis of whole-mount skeletal preparations. Whole-mount skeletal staining permits evaluation of the shapes and sizes of skeletal elements in their appropriate locations. The technique
    Author: Diana Rigueur…
    Published: 2014
    DOI: 10.1007/978-1-62…
    National Center for Biotechnology Information
    Mouse Mammary Gland Whole Mount Preparation and Analysis
    The mammary gland undergoes extensive remodeling during pregnancy and is also subject to neoplastic processes both of which result in histological changes of the gland epithelial structure. Since the mammary tree is a complex three-dimensional structure
    Author: Cornelia Tolg, …
    Published: 2018/07/07
    Publication: Bio Proto…
    PubMed
    Whole-Mount Immunofluorescence Staining of Early Mouse Embryos
    Immunofluorescence staining enables the visualization of protein expression at a cellular or even sub-nuclear level. Whole-mount staining preserves the three-dimensional spatial information in biological samples allowing a comprehensive interpretation
    Author: Frederick C K …
    Published: 2021
    Keywords: Embryo; Im…
    PubMed
    Fluorescence-based Single-cell Analysis of Whole-mount-stained and Cleared Microtissues and Organoids for High Throughput Screening
    Three-dimensional (3D) cell culture, especially in the form of organ-like microtissues ("organoids"), has emerged as a novel tool potentially mimicking human tissue biology more closely than standard two-dimensional culture. Typically, tissue sectioning
    Author: Henrik Renner
    Published: 2021/06/20
    Keywords: (High-conte…
    PubMed
    iDISCO: a simple, rapid method to immunolabel large tissue samples for volume imaging
    The visualization of molecularly labeled structures within large intact tissues in three dimensions is an area of intense focus. We describe a simple, rapid, and inexpensive method, iDISCO, that permits whole-mount immunolabeling with volume imaging of
    Author: Nicolas Renier
    Published: 2014/11/06
    Keywords: National In…
    PubMed
    Whole-Mount Immunofluorescence Staining, Confocal Imaging and 3D Reconstruction of the Sinoatrial and Atrioventricular Node in the Mouse
    The electrical signal physiologically generated by pacemaker cells in the sinoatrial node (SAN) is conducted through the conduction system, which includes the atrioventricular node (AVN), to allow excitation and contraction of the whole heart. Any dysfunction
    Author: Ruibing Xia
    Published: 2020/12/22
    Keywords: National In…
    National Center for Biotechnology Information
    Click histochemistry for whole-mount staining of brain structures
    Open in a separate window Method name: Whole-mount click histochemistry Keywords: Click chemistry, 5-ethynyl-2'-deoxyuridine, Whole-mount histochemistry, Neurogenesis, Cell division, Cell migration, Hippocampus, 3D imaging
    Author: Alexander A. L…
    Published: 2019
    Publication: MethodsX…
    PubMed
    Visualization of 3D White Adipose Tissue Structure Using Whole-mount Staining
    Adipose tissue is an important metabolic organ with high plasticity and is responsive to environmental stimuli and nutrient status. As such, various techniques have been developed to study the morphology and biology of adipose tissue. However, conventional
    Author: Yanqing Jiang
    Published: 2018/11/17
    Keywords: National In…
    National Center for Biotechnology Information
    A novel protocol of whole mount electro-immunofluorescence staining
    Purpose To develop a new method of whole mount immunostaining that improves the penetration of staining reagents into the cornea and decreases non-specific binding and background. Methods Adult mouse corneas were fixed overnight in 4% paraformaldehyde
    Author: Hongshan Liu…
    Published: 2009
    Publication: Mol Vis. 2…
    PubMed
    Whole-mount staining of mouse colorectal cancer organoids and fibroblast-organoid co-cultures
    Imaging organoid culture provides an excellent tool for studying complex diseases such as cancer. However, retaining the morphology of intact organoids for immunolabeling has been challenging. Here, we describe a protocol for immunofluorescence staining
    Author: Anxo Martinez …
    Published: 2023 Apr
    Keywords: Cancer; Ce…
  7. MilliporeSigma
    https://www.sigmaaldrich.com/US/en/technical...

    Protocol Guide: Immunofluorescent Staining of Whole-Mount …

  8. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5384832

    Whole-Mount Skeletal Staining - PMC - National Center for …

    Tags:
    Publish Year:2014
    Diana Rigueur, Karen M. Lyons
    Published:2014
  9. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8328583

    Mouse Mammary Gland Whole Mount Preparation and Analysis

    Tags:
    Whole Mount Tissue Protocol
    Publish Year:2018
    Mammary gland
  10. Plant Methods
    https://plantmethods.biomedcentral.com/articles/10...

    Protocol: an improved and universal procedure for whole-mount ...

    Tags:
    Publish Year:2015
    Protocol
  11. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8792446

    Protocol for whole-mount X-gal staining combined with tissue …

    Tags:
    10.1016/j.xpro.2022.101127
    STAR Protoc. 2022 Mar 18; 3(1): 101127.
    CUBIC TCP
  12. Abcam
    https://docs.abcam.com/pdf/protocols/Whole_mount_fluorescent_ihc.pdf
    [PDF]

    Whole mount - Abcam

    Tags:
    Tissue
    Whole Mount Embryo Stain
  13. Springer
    https://link.springer.com/article/10.1007/s10911-013-9285-5

    A Whole-mount Immunofluorescence Protocol for Three

    Tags:
    Mammary gland
    Naoko Kogata, Beatrice A. Howard
    Publish Year:2013
    E 11
  14. Nature
    https://www.nature.com/articles/nprot.2011.441

    Whole-mount three-dimensional imaging of internally localized ...

    Tags:
    Whole Mount Staining
    Whole Mount Imaging
    Publish Year:2012
  15. PubMed
    https://pubmed.ncbi.nlm.nih.gov/32944908

    Whole-Mount Immunofluorescence Staining of Early Mouse …

    Tags:
    Laboratory Techniques in Biology
    Immunofluorescence Staining
    Publish Year:2021
  16. Fralin Biomedical Research Institute at VTC
    https://fbri.vtc.vt.edu/content/dam/fbri_vtc_vt_edu...
    [PDF]

    Tissue whole mount immunostaining - Virginia Tech

    Tags:
    Tissue
    Whole Mount Imaging
  17. Nature
    https://www.nature.com/articles/s41596-020-0376-7

    Combined whole-mount fluorescence in situ hybridization and

    Tags:
    Laboratory Techniques in Biology
    Publish Year:2020
    Fish Staining
  18. Cold Spring Harbor Protocols
    https://cshprotocols.cshlp.org/content/2008/1/pdb.prot4820.full

    Immunohistochemistry of Whole-Mount Mouse Embryos - CSH …

    Tags:
    Cells
    Alex Joyner
  19. PubMed
    https://pubmed.ncbi.nlm.nih.gov/34262994

    Fluorescence-based Single-cell Analysis of Whole-mount-stained …

    Tags:
    Publish Year:2021
    Zoids: Chaotic Century
  20. PubMed
    https://pubmed.ncbi.nlm.nih.gov/25417164

    iDISCO: a simple, rapid method to immunolabel large tissue

    Tags:
    Publish Year:2014
  21. STEMCELL Technologies
    https://www.stemcell.com/how-to-perform-immunocyto...

    ICC Staining Protocol for Monolayer or Air-Liquid Interface

    Tags:
    Cells
    Culture
  22. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10192636

    Whole-mount immunofluorescence staining of blood and …

    Tags:
    Immunofluorescence Staining Protocol
    10.1016/j.xpro.2023.102310
  23. PubMed
    https://pubmed.ncbi.nlm.nih.gov/33427243

    Whole-Mount Immunofluorescence Staining, Confocal Imaging …

    Tags:
    Whole Mount Staining
    Whole Mount Tissue Protocol
    Publish Year:2020
  24. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6812327

    Click histochemistry for whole-mount staining of brain structures

    Tags:
    Histochemistry Staining
    Publish Year:2019
    10.1016/j.mex.2019.09.011
  25. United States Environmental Protection Agency (.gov)
    https://www3.epa.gov/pesticides/chem_search/ppls/...
    [PDF]

    Deanna Mansueto Senior Regulatory Affairs Associate …

  26. Nature
    https://www.nature.com/articles/s42255-024-01105-9

    Cancer tissue of origin constrains the growth and metabolism of ...

    Tags:
    Whole Mount Staining
    Cancer
  27. PubMed
    https://pubmed.ncbi.nlm.nih.gov/30507917

    Visualization of 3D White Adipose Tissue Structure Using Whole …

    Tags:
    Publish Year:2018
    White County
  28. Science | AAAS
    https://www.science.org/doi/epdf/10.1126/sciadv.adq6082
    [PDF]

    PLANT SCIENCE copyright © 2024 the Cytokinin and ALOG …

    Tags:
    Science
    Cytokinin
  29. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2650717

    A novel protocol of whole mount electro-immunofluorescence …

    Tags:
    Whole Mount Staining
    Immunofluorescence Staining Protocol
    Publish Year:2009
  30. PubMed
    https://pubmed.ncbi.nlm.nih.gov/37083323

    Whole-mount staining of mouse colorectal cancer organoids and …

    Tags:
    Colorectal cancer
    Organoid
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