www.abcam.com/tissue-processing gradual dehydration in alcohol series and xylene to allow parafin penetration. … See more

Block with sera or a protein to prevent non-specific antibody binding and reduce background and potentially false positive results. We need to perform blocking with sera or a protein blocking reagent to prevent no… See more

For enzymatic detection methods, block endogenous enzymes so as not to confound your results. Consider blocking endogenous enzymes after incubating with your primary antibody, as treatments like H2O2 can … See more

A critical decision when designing an IHC experiment is primary antibody selection since successful immunostaining relies on our primary antibody specifically binding the target antigen. See more

Before choosing our primary, we need to consider whether we plan to use direct or indirect detection methods. The antibody can be detected either directly, through a label that is directly conjugated to the primary ant… See more