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- Nuclear staining is a technique used to stain cell nuclei only1. It is used to confirm the shape or location of cells1. Cell-permeant nucleic acid stains make it possible to stain live cells or tissues that have been minimally processed2. Examples of cell-permeant nuclear stains include Hoechst stains and SYTO® stains3. Live cell nuclear stains can be used either for cell tracking, or as counterstains with other fluorescent dyes or cell reporters4.
Learn more:✕This summary was generated using AI based on multiple online sources. To view the original source information, use the "Learn more" links.Nuclear staining refers to staining of cell nuclei only. Cultured cells are generally colorless and transparent, so they are often observed with a phase contrast microscope while alive. However, to clearly confirm the shape or location of cells, staining may be performed.www.healthcare.nikon.com/en/ss/cell-image-lab/glo…Cell-permeant nucleic acid stains make it possible to stain live cells or tissues that have been minimally processed. Nuclear staining can reveal the natural location of cells in tissues and can provide a means to follow nuclear changes throughout processes such as mitosis and apoptosis (Assays for Apoptosis—Section 15.5).www.thermofisher.com/us/en/home/references/mol…Cell-permeant nuclear stains can be used to label nuclei in live cells that have intact, nonpermeable plasma membranes. These dyes will also stain nuclei of cells with compromised membranes such as dead cells or cells that have been fixed and/or permeabilized. Examples of cell-permeant nuclear stains include Hoechst stains and SYTO® stains.www.thermofisher.com/us/en/home/life-science/cel…Containing nearly all of the cell's genetic material, the cell nucleus is organized in chromosomes, formed from the complexation of long linear DNA with proteins. Live cell nuclear stains can be used either for cell tracking, or as counterstains with other fluorescent dyes or cell reporters.www.sigmaaldrich.com/US/en/technical-documents… - People also ask
NucSpot® Live Cell Nuclear Stains - Biotium
Mar 9, 2017 · NucSpot® Live Cell Nuclear Stains are cell membrane-permeant DNA dyes that specifically stain nuclei in live or fixed cells. They have excellent specificity for DNA without the need for a wash step, with low toxicity for live cell imaging.
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See results only from biotium.comNuclear Stains
NucSpot® Live Nuclear Stains are low-toxicity stains that offe…
Hoechst & DAPI Staining Pro…
Hoechst and DAPI are popular blue fluorescent, nuclear-spec…
NucSpot® Nuclear Stains
Selectively stains dead cells in live culture. No wash required…
NucBlue™ Live ReadyProbes™ Reagent (Hoechst 33342)
Hoechst 33342 is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to DNA. With NucBlue Live ReadyProbes Reagent we have formulated this …
Nuclear Labeling | Thermo Fisher Scientific - US
- TO-PRO®-3 stain exhibits a bright signal in the nucleus and a dimmer signal …
- HUVECs labeled with NucRed™ Live, a cell-permeant nuclear stain.
- Live and dead HEPG2 cells stained using the LIVE/DEAD® Cell Imaging Kit. …
- Nuclear staining of fixed and permeabilized U2OS cells using NucBlue™ …
- Example of incomplete live-cell nuclear staining. A proportion of the nuclei in …
Live Cell Fluorescent Organelle Dyes - MilliporeSigma
Live cell nuclear stains can be used either for cell tracking, or as counterstains with other fluorescent dyes or cell reporters. We offer dyes with several color options, including long-wavelength, far-red nuclear dyes.
Nuclear Stains - Biotium
NucSpot® Live Nuclear Stains are low-toxicity stains that offer nuclear staining for real-time imaging. No-wash is required and staining is stable for up to 72 hours. NucSpot® Live 488 has green fluorescence (Ex/Em 500/515 nm), while …
Hoechst 33342 Protocol for Imaging - Thermo Fisher Scientific
Nuclear counterstain for fluorescence microscopy. Invitrogen Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to …
Hoechst & DAPI Staining Protocols - Cell Staining with ... - Biotium
Hoechst and DAPI are popular blue fluorescent, nuclear-specific dyes that can be used to stain live or fixed cells. Both DAPI and Hoechst are minor-groove binding dyes with a preference for …
Live Cell Imaging Reagents - MilliporeSigma
Live cell imaging in real time allows scientists to monitor dynamic cellular events that otherwise escape observation using traditional endpoint techniques like fixed imaging and PCR that capture only a static moment in time.
Differential nuclear staining assay for high-throughput screening …
Here we introduce a Differential Nuclear Staining (DNS) assay adapted to live-cell imaging for high throughput screening (HTS) that utilizes two fluorescent DNA intercalators, Hoechst …
SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy
Oct 1, 2015 · The ideal DNA stain for live-cell imaging would (i) show high selectivity, (ii) show minimal toxicity, (iii) be fluorogenic for wash-free imaging, (iv) be applicable in different cell …
BioTracker 488 Green Nuclear Dye | SCT120 - Merck
The BioTracker 488 Green Nuclear Dye is a cell-membrane permeable green fluorescent DNA dye that specifically stains nuclei in live or fixed cells. It has excellent specificity for DNA without the need for a wash step, and it has low …
Nuclear Labeling Reagent | See the Difference | Sartorius
Incucyte ® Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling was formulated explicitly for cell labeling of living mammalian cells. The dye is a cell-permeable DNA strain that specifically …
Nuclear Staining | Thermo Fisher Scientific - US
Incubate for 5–15 minutes at room temperature or 37°C for most nuclear dyes; some live-cell dyes may give you a better signal with longer incubation times. 5: Optional: For live-cell …
NucSpot® Live 650 1000X in DMSO live cell nuclear stain | VWR
NucSpot® Live Cell Nuclear Stains are cell-membrane permeable DNA dyes that specifically stains nuclei in live or fixed cells. They have excellent specificity for DNA without the need for …
BioTracker 488 Green Nuclear Dye Live Cell Imaging Millipore
The BioTracker 488 Green Nuclear Dye is a cell-membrane permeable green fluorescent DNA dye that specifically stains nuclei in live or fixed cells. It has excellent specificity for DNA …
RNA-Selective, Live Cell Imaging Probes for Studying Nuclear …
Jun 23, 2006 · RNA-Selective, Live Cell Imaging Probes for Studying Nuclear Structure and Function. Summary. The higher-order structural organization of the cell nucleus reflects the …
NucSpot® Nuclear Stains - Biotium
Selectively stains dead cells in live culture. No wash required. Color options for FITC, Cy®3, PE, Texas Red®, Cy®5, APC, Cy®5.5, and Cy®7 channels. NucSpot® Stains have minimal …
Fluorescent Cell Stains for Organelles & Cellular Structures
NucSpot® Live Nuclear Stains specifically stain nuclei in live or fixed cells with no need for washing. NucSpot® Live 488 has green fluorescence (Ex/Em 500/515 nm), while NucSpot® …
Do you have any dyes that can stain the nuclei in live cells? Are …
Aug 3, 2021 · Answer. DAPI and Hoechst 33258, 33342 and 34580 can be used to stain nuclei in either live or fixed cells. Unfortunately, when exposed to UV light during imaging, the blue dyes …
Probes for the Nucleus—Section 12.5 - Thermo Fisher Scientific
Nuclear staining by YO-PRO-1 dye has provided a method to identify individual cells within single live, perfused mesentery microvessels. Staining with the TOTO-1 ( T3600) and YOYO …
Invitrogen NucBlue Live ReadyProbes Reagent (Hoechst 33342) …
Hoechst 33342 is perhaps the most ubiquitous nuclear fluorescence stain for live and fixed cells and tissue sections. The cell permeable stain binds preferentially to AT regions of DNA. It is …
BioTracker 488 Green Nuclear Dye | SCT120 - EMD Millipore
BioTracker 488 Green Nuclear Dye Live cell imaging green nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst …
BioTracker 650 Red Nuclear Dye ++ Photostability Millipore
Live cell imaging red nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342. Sign In to View Organizational & …
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