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- To stain live cells, you can follow the following protocol1:
- Add the dye to complete culture medium. We recommend using Hoechst dyes at 1 ug/mL or DAPI at 10 ug/mL.
- Remove culture medium from the cells and replace with medium containing dye.
- Incubate cells at room temperature or 37°C for 5-15 minutes, then image. Note: Washing is not necessary for specific staining, but nuclear staining is stable after washing.
Learn more:âś•This summary was generated using AI based on multiple online sources. To view the original source information, use the "Learn more" links.Considerations for Staining Live Cells
- Add the dye to complete culture medium. We recommend using Hoechst dyes at 1 ug/mL or DAPI at 10 ug/mL. ...
- Remove culture medium from the cells and replace with medium containing dye.
biotium.com/tech-tips/protocol-staining-cells-with-h…Staining protocol 1. Discard the cell culture medium by inverting the slide and gently tapping it on a paper towel to remove the remaining medium. 2. ™Fix the cells with 4% formaldehyde (diluted in 1X PBS— prepare fresh) for 10 min at room temperature (fixation time can be increased to 20 min depending on the cell line).
assets.thermofisher.com/TFS-Assets/BID/Referenc… - People also ask
Live-Cell Imaging | Thermo Fisher Scientific - US
Learn the simple steps to stain your live cells for dynamic imaging experiments. What is fluorescence live-cell imaging? Fluorescence imaging of live cells is used to observe dynamic cellular processes and track cellular biomolecules and structures over time.
See results only from thermofisher.comHoechst 33342 Protocol for I…
1. Culture cells in an appropriate medium and vessel for fluorescence microscopy. …
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The Thermo ScientificTM NuncTM Lab-TekTM II CC2 Chamber Slide System …
5 steps to live-cell imagin…
5 steps to live-cell imaging. Follow this guide to capture the best possible …
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Live cells stained with antibodies can be sorted based on unique staining …
BestProtocols: Viability Stai…
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Live Cell Fluorescent Organelle Dyes and Stains - MilliporeSigma
Our live cell fluorescent organelle dyes permit the selective staining of specific organelles without increased cytotoxicity. Organelle staining in live cells can help researchers to confirm the …
Hoechst & DAPI Staining Protocols - Cell Staining with ... - Biotium
See more on biotium.comHoechst and DAPI are popular blue fluorescent, nuclear-specific dyes that can be used to stain live or fixed cells. Both DAPI and Hoechst are minor-groove binding dyes with a preference for A/T-rich regions of DNA over G/C-rich DNA. The dyes have minimal fluorescence in solution, but become brightly fluorescent upon bindi…- Estimated Reading Time: 7 mins
LIVE/DEAD Fixable Dead Cell Stains Protocol
The Invitrogen LIVE/DEAD fixable dead cell stains distinguish between live and dead cells in flow cytometry. The dyes covalently bind to intracellular and extracellular amines, allowing the staining pattern to be preserved following …
Procedure for staining of cell cultures using immunofluorescence. ICC and IF protocol. Preparing the slide. Coat coverslips with polyethylineimine or poly-L-lysine for 1 h at room temperature. …
An introduction to Performing Immunofluorescence Staining
Here, we describe preparation of specimens preserved in different types of media and step-by-step methods for both direct and indirect immunofluorescence staining. Keywords: …
5 steps to live-cell imaging. Follow this guide to capture the best possible images. Introduction. Fluorescence imaging of live cells is a powerful approach to the study of dynamic cellular …
Protocols for staining vary depending on the assay. This is a generalized cell surface staining protocol for live cell sorting to use as a guide. Sorting Buffers: Hank’s Balanced Salt Solution …
Choosing the Right Stain for Your Particular Biological Question. Fluorescence labeling has revolutionized live-cell imaging. To get the best live images, however, there are several factors …
Protocol: Immunofluorescence Staining of Cells for Microscopy
This is our basic protocol for staining adherent cells in dishes or cells grown on coverslips. Materials required: PBS or HBSS (buffer with Ca 2+ /Mg 2+ may be optimal for adherent cells)
e perfect protocol of how to fix, stain, and analyze cells. Producing beautiful, eye-catching, ready-to-publish IF stainings requires optimization of the best conditions for each single step of the …
The Thermo ScientificTM NuncTM Lab-TekTM II CC2 Chamber Slide System allows for growth and staining of cells on a single surface. A chamber slide consists of a removable polystyrene …
Hoechst 33342 Protocol for Imaging - Thermo Fisher Scientific
1. Culture cells in an appropriate medium and vessel for fluorescence microscopy. 2. Prepare the Hoechst staining solution by diluting the Hoechst stock solution 1:2,000 in PBS. 3. Remove the …
Immunofluorescence Labeling of Cells - MilliporeSigma
Cell staining can be divided into four steps: cell preparation, fixation, application of antibody, and evaluation. As a first step, cells to be stained are attached to a solid support to allow easy …
Live cell staining dyes - Abcam
Jan 8, 2023 · Analyze your cells of interest for hours or days with cell-tracking dyes that are retained in living cells. These dyes are hydrophobic compounds that can permeate through the …
Live Cell Fluorescence Microscopy to Observe Essential …
Protocols for cell labeling can be applied to protein depletion mutants to identify defects in membrane structure, cell wall biogenesis, or chromosome segregation. Furthermore, time …
BestProtocols: Staining Cell Surface Targets for Flow Cytometry
Live cells stained with antibodies can be sorted based on unique staining patterns and used for additional experiments. Staining cell surface targets protocols. Protocol A: Cell Suspensions. …
Flow Cytometry Live Cell Protocol
Flow Cytometry Live Cell Protocol. IMPORTANT: Please see the product-specific Flow Cytometry protocol on the product webpage to confirm whether it may be used with live cells, and for …
A diet-dependent host metabolite shapes the gut microbiota
2 days ago · All animal experiments were conducted under protocols (AN200526 and AN199820) ... Fisher Scientific; cat# BDB563331), and live/dead staining was performed using aqua …
BestProtocols: Viability Staining Protocol for Flow Cytometry
Introduction. Viability staining is an essential component of any flow cytometry experiment. Dead cells can compromise the integrity of the data by non-specifically binding antibodies; therefore …
BestProtocols: Staining Intracellular Antigens for Flow Cytometry
Introduction. A modification of the basic immunofluorescent staining and flow cytometric analysis protocol can be used for the simultaneous analysis of surface molecules and intracellular …
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