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- Here is a protocol for the PicoGreen assay12:
- Protect PicoGreen from light.
- Dilute PicoGreen 1:200 in 1x TE buffer.
- Add 100 μl of the PicoGreen working solution into each well of a white 96-well plate.
- Pipette 100 μl of blank, DNA standard dilutions and samples into the wells of the plate with a defined number of replicates.
- Swirl the bottle to mix the PicoGreen.
- An additional 1 ml TE with 5 ul PicoGreen may need to be prepared for the standards.
- Add PicoGreen to DNA samples.
Learn more:✕This summary was generated using AI based on multiple online sources. To view the original source information, use the "Learn more" links.PicoGreen Should be protected from light. 100 ul of diluted PicoGreen is needed per sample/standard. For each DNA plate to be quantified add: 10 ml 1x TE 50 ul PicoGreen Mix by swirling bottle. (An additional 1 ml TE with 5 ul PicoGreen may need to be prepared for the standards.) Add PicoGreen to DNA sampleswww.k-state.edu/igenomics/equipment/documents/…Procedure for 96-well plate assay Pipette 100 μl of blank, DNA standard dilutions and samples into the wells of white 96-well plate with a defined number of replicates (having at least 2- 3 replicates is recommended). Add 100 μl of the PicoGreen working solution into each well.assets.thermofisher.com/TFS-Assets/LCD/Applicati… 
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Using the Quant-iT ™ PicoGreen dsDNA Reagent and the recommended assay protocol, researchers can quantitate dsDNA in the presence of equimolar concentrations of ssDNA and …
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- The Quant-iTTM PicoGreen® reagent supplied in the kits is exactly the same as the reagent sold separately.
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agarose. The assay protocol has been developed to minimize the fluorescence contribution of RNA and single-stranded DNA (ssDNA). Using the PicoGreen dsDNA Quantitation Reagent …
STANDARD OPERATING PROCEDURE (SOP) FOR PICOGREEN DNA QUANTIFICATION (MANUAL) I. SCOPE AND PURPOSE PicoGreen is an ultra-sensitive fluorescent nucleic acid …
PicoGreen® dsDNA Quantitation Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) in molecular biology procedures.
Prepare PicoGreen in a foil cover bottle. PicoGreen Should be protected from light. 100 ul of diluted PicoGreen is needed per sample/standard. 50 ul PicoGreen Mix by swirling bottle. (An …
PicoGreen Assay Protocol
The PicoGreen assay is useful for quantifying the amount of double-stranded DNA (dsDNA) that is present in a given sample. The measured amount of dsDNA can then be used as a marker for …
Apr 12, 2011 · The PicoGreen dsDNA assay is used for detection and quantitation of double-stranded DNA (dsDNA) in an aqueous solution. This SOP will be used by Process Analytics …
A 1:200 dilution of PicoGreen reagent is prepared by adding 10 μl of PicoGreen per 2 mL of 1X TE buffer. You will need 100 ul diluted PicoGreen per well containing 100 ul sample.
DNA quantification in plates (picogreen protocol). Allow the Quant-iT PicoGreen reagent to warm to room temperature before opening the vial. PicoGreen is light sensitive; while thawing, wrap …
In combination with Thermo Scientific Fluoroskan Ascent or Fluoroskan Ascent FL, the PicoGreen reagent provides rapid and easy determination of dsDNA, offering a wide dynamic range. …
Incubate for 2-5 minute in dark before reading in BMG Labtech FLUOstar. Put plate inside of plate reader then click ‘Measure’ icon. Select highest standard well and click ‘Gain Adjustment’ icon …
PicoGreen® dsDNA Quantitation Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) in molecular biology procedures. Turner …
PicoGreen Protocol
Add 50 ul diluted PicoGreen reagent (from step 2) to each well for every sample and standard. Standards: Add 50 ul of each standard to wells in column 1 as indicated in step 3b.
Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents
Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA …
PicoGreen Assay
Oct 18, 2024 · The protocol for checking quantification of DNA using the PicoGreen Assay on the Synergy HTX Plate Reader. Important: PicoGreen vs. Qubit When running a full plate of …
Protocol for dsDNA Quantitation using PicoGreen - The Lab Rat
The assay protocol has been developed to minimize the fluorescence contribution of RNA and single-stranded DNA (ssDNA). Using the PicoGreen dsDNA Quantitation Reagent and the TD …
Protocol for DNA Quantitation Using PicoGreen - The Lab Rat
The assay protocol has been developed to minimize the fluorescence contribution of RNA and single-stranded DNA (ssDNA). Using the PicoGreen dsDNA Quantitation Reagent and the …
RiboGreen - AAT Bioquest
Both Qubit and RiboGreen assays generally outperform UV absorbance methods at lower concentration ranges, reflecting the advantage of fluorescence-based approaches when …
molecprot_picogreenassay – Lotterhos Lab Protocols
The protocol for checking quantification of DNA using the PicoGreen Assay on the Synergy HTX Plate Reader. Important: PicoGreen vs. Quibit Although the PicoGreen and Qubit both use …
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