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- Quant-iTTM PicoGreen® dsDNA reagent is an ultra sensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) in solution. Detecting and quantitating small amounts of DNA is extremely important in a wide variety of biological applications.Learn more:Quant-iTTM PicoGreen® dsDNA reagent is an ultra sensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) in solution. Detecting and quantitating small amounts of DNA is extremely important in a wide variety of biological applications.tools.thermofisher.com/content/sfs/manuals/mp075…The Quant-iTTM PicoGreenTM dsDNA Reagent enables researchers to quantitate as little as 250 pg/mL of dsDNA (50 pg dsDNA in a 200 μL assay volume) with a fluorescence microplate reader using fluorescein excitation and emission wavelengths.assets.thermofisher.com/TFS-Assets/LSG/manuals…The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA quantitation, measuring dsDNA in complex mixtures, and viral DNA quantitation. For Research Use Only. Not for use in diagnostic procedures.www.thermofisher.com/order/catalog/product/P7589The Quant-iTTM PicoGreen® dsDNA Reagent is an ultra-sensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) in molecular biology procedures.www.promega.com/~/media/files/products%20and…We use the Quant-iTTM PicoGreen® dsDNA kit for quantification of both extracted and amplified DNA. The Quant-iTTM PicoGreen® dsDNA reagent is a proprietary, asymmetrical cyanine dye. Free dye does not fluoresce, but upon binding to dsDNA it exhibits a >1000-fold fluorescence enhancement.www.researchgate.net/publication/370672395_DN…
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Quant-iT™ PicoGreen™ dsDNA Assay Kits and …
The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA quantitation, measuring dsDNA in complex mixtures, and viral …
PicoGreen® dsDNA Quantitation Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) in molecular biology procedures.
- The Quant-iTTM PicoGreen® reagent supplied in the kits is exactly the same as the reagent sold separately.
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The Quant-iTTM PicoGreen® dsDNA Reagent is an ultra-sensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) in molecular biology procedures.
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The PicoGreen® dye is a fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA).
PicoGreen Protocol
Dilute PicoGreen reagent 1:200 in a foil-wrapped 50 ml tube. * Final concentration after diluted PicoGreen reagent added at 1:1 ratio. Add 50 ul diluted PicoGreen reagent (from step 2) to …
Protocol Citation: Jenn Coughlan, Jen Modliszewski, Cathy Rushworth 2020. DNA quantification in plates (picogreen protocol). Allow the Quant-iT PicoGreen reagent to warm to room …
A 1:200 dilution of PicoGreen reagent is prepared by adding 10 μl of PicoGreen per 2 mL of 1X TE buffer. You will need 100 ul diluted PicoGreen per well containing 100 ul sample.
Protocol for dsDNA Quantitation using PicoGreen
PicoGreen dsDNA Quantitation Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) in solution. Detecting and quantitating small amounts of DNA is extremely important in a wide …
Dilute the PicoGreen reagent 1:200 by adding 5 µL of stock dye per 1 mL 1X TE. PicoGreen is a light sensitive dye and should be protected from light when possible. Keep this reagent in the …
Dilute the PicoGreen reagent 1:200 with TE buffer in a plastic tube. Protect the working solution from light and use it within a few hours after preparation. 100 μl/well of diluted reagent is …
PicoGreen® dsDNA Quantitation Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) in molecular biology procedures.
The Quant-iTTM PicoGreenTM dsDNA Reagent enables researchers to quantitate as little as 250 pg/mL of dsDNA (50 pg dsDNA in a 200 μL assay volume) with a fluorescence microplate …
Protocol for DNA Quantitation Using PicoGreen - The Lab Rat
PicoGreen dsDNA Quantitation Reagent is an ultra-sensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) in molecular biological procedures such as cDNA …
Apr 12, 2011 · Because the PicoGreen dye is an extremely sensitive detection reagent for dsDNA, it is imperative that the TE buffer is free of contaminating nucleic acids. Note: The 20X …
On the day of the experiment, prepare a 2x working solution of the PicoGreen® Reagent by making a 1:200 dilution of the concentrated dye solution in 1xTE (10 mM Tris-HCl, 1 mM …
DNA quantification with PicoGreen - protocols.io
May 23, 2023 · The Quant-iT™ PicoGreen® dsDNA reagent is a proprietary, asymmetrical cyanine dye. Free dye does not fluoresce, but upon binding to dsDNA it exhibits a >1000-fold …
We use the Quant-iTTM PicoGreen® dsDNA kit for quantification of both extracted and amplified DNA. The Quant-iTTM PicoGreen® dsDNA reagent is a proprietary, asymmetrical cyanine …
This translates into DNA sample concentrations of 0.05-5 ng μl-1 and 1-200 ng μl-1 in the low-range and high-range assays, respectively. Samples with higher DNA concentration need to …
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