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Aseptic Laboratory Techniques: Plating Methods - PMC
Pour-plate technique. (A) A small volume of sample (between 0.1 to 1.0 ml) is dispensed aseptically into an empty but sterile Petri dish with using a 5.0 ml serological pipette. (B) Melted agar equilibrated to a temperature of …
Other content from pmc.ncbi.nlm.nih.govImprovement of the Pour Plate Method by Separate …
Jan 10, 2023 · A flexible modular system could be designed for the pour plate method, with automatic control of temperature and fluid dynamics properties for pouring, sample mixing, dilution, and sterile plate incubation management.
Incubation temperature and culture medium formulation impact …
Jul 1, 2021 · In the present study, we assessed the accuracy of several pour-plate techniques for the enumeration of a mixed-species Bacillus assemblage. Tested modifications included a …
- Author: Taliesin Lenhart, John Gorsuch
- Publish Year: 2021
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Pour Plate Method- Definition, Principle, Procedure, …
Aug 27, 2022 · What is Pour Plate Method? The pour plate method is a microbiological laboratory technique for isolating and counting the viable …
- Estimated Reading Time: 9 mins
pour plate and spread plate methods Raymond H. Taylor, Martin J. Allen, and Edwin E. Geldreich Water samples collected during routine flushing of dead-end mains were analyzed for bacterial …
Improvement of the Pour Plate Method by Separate …
Jan 10, 2023 · In the era of rapid microbiological methods, there is a need to improve long-established culture techniques. Drawbacks of the pour plate method include having to melt each medium separately...
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The pour plate method allows the plating of higher volumes of sample (1 mL) by dis- pensing a liquid inoculum onto an empty petri dish, which is then flooded with a molten medium (12, 13), …
Improvement of the Pour Plate Method by Separate Sterilization …
Dec 8, 2022 · The pour plate method allows the plating of higher volumes of sample (1 mL) by dispensing a liquid inoculum onto an empty petri dish, which is then flooded with a molten …
Incubation temperature and culture medium formulation impact …
Jul 1, 2021 · In the present study, we assessed the accuracy of several pour-plate techniques for the enumeration of a mixed-species Bacillus assemblage. Tested modifications included a …
Improvement of the Pour Plate Method by Separate Sterilization …
Feb 14, 2023 · Sterilizing agar separately from the other components of the medium and reducing the agar concentration to 10 g/L can improve microorganism growth and enhance the …
Aseptic Laboratory Techniques: Plating Methods
May 11, 2012 · Isolate single bacterial colonies by the streak-plating method. Use pour-plating and spread-plating methods to determine the concentration of bacteria. Perform soft agar overlays when working...
Comparison of the Pour, Spread, and Drop Plate Methods for …
Inoculants prepared with presterilized peat were enumerated by the pour, spread, and drop plate techniques. Results indicated that the three plating methods were interchangeable. The drop …
Standard plate count: A comparison of pour plate and spread …
Jan 1, 1983 · Water samples collected during routine flushing of dead-end mains were analyzed for bacterial count by using both pour plate and spread plate procedures. Results from these …
Incubation temperature and culture medium formulation impact …
In the present study, we assessed the accuracy of several pour-plate techniques for the enumeration of a mixed-species Bacillus assemblage. Tested modifications included a …
Improvement of the Pour Plate Method by Separate Sterilization …
A flexible modular system could be designed for the pour plate method, with automatic control of temperature and fluid dynamics properties for pouring, sample mixing, dilution, and sterile …
Pour Plate Method: Procedure, Uses, (Dis) Advantages
Pour plate method is usually the method of choice for counting the number of colony-forming bacteria present in a liquid specimen. Because the sample is mixed with the molten agar …
Mechanical Preparation of Pour Plates for Viable Bacterial …
Aug 1, 1973 · J.G.Bradshaw, D.W.Francis, J.T.Peeler, J.E.Leslie, R.M.Twedt, R.B.ReadJr. A machine for making bacterial pour plates to determine the viable bacteria counts of milk …
Conclusion: The Pour plate technique was observed to be the sensitive method in identifying significant bacteriuria compared to standard loop technique. Escherichia coli showed the …
Evaluation of Measurement Uncertainty from Pour Plate Method …
Apr 25, 2022 · In this study, we aimed to evaluate the MU in total bacteria counts (TBC) in water. Experiments were carried out using Pour Plate Method. The bottom-up approach was used to …
Nov 2, 2022 · In this study, we aimed to evaluate the MU in total bacteria counts (TBC) in water. Experiments were carried out using Pour Plate Method. The bottom-up approach was used to …
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