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- Studies of retina whole mount staining
Optimized Protocol for Retinal Wholemount Preparation for …
WEBDec 13, 2013 — Demonstrated here are two representative experiments that benefit from the use of a hydrophilized PTFE membrane to support wholemount retina during staining procedures.
- Author: Elena Ivanova, Abduqodir H Toychiev, Christopher W Yee, Botir T Sagdullaev
- DOI: 10.3791/51018
- Publish Year: 2013
- Publication: J Vis Exp. 2013; (82): 51018.
See results only from ncbi.nlm.nih.govNonlinear Interactions betwe…
Whole-cell recordings were made from BCs using patch pipettes filled with an …
Immunostaining of Whole-M…
Here, we adapted the CLARITY method for whole-mount mouse retinas by …
Dissection and Whole Moun…
Here we provide a detailed protocol for whole mount staining of mouse retina. …
Whole Mount Immunofluo…
Here we provide a detailed description of a reliable and technically straight forward …
A New Method to Visualize t…
An RPE/retina whole mount staining protocol provided better morphology of …
Mouse Retinal Whole Mo…
This protocol describes the steps involved in the whole mount processing of mouse …
Non-separate Mouse Sclero…
Here, we describe steps of both non-pigmented and pigmented mouse …
Retinal Cryo-sections, Wh…
We describe retinal microvascular pericyte immunohistochemical staining in cryo …
Immunostaining of Whole-Mount Retinas with the CLARITY …
WEBMar 6, 2021 — Here, we adapted the CLARITY method for whole-mount mouse retinas by polymerizing them with an acrylamide monomer to form a nanoporous hydrogel and …
- Author: Elizabeth J Alessio, Dao-Qi Zhang
- Publish Year: 2021
Dissection and Whole Mount Staining of Retina from Neonatal Mice
WEBOct 10, 2018 — Here we provide a detailed protocol for whole mount staining of mouse retina. This protocol was used to analyze retinal angiogenesis in newborn mice ( …
Whole Mount Immunofluorescent Staining of the Neonatal Mouse …
WEBJul 9, 2013 — Here we provide a detailed description of a reliable and technically straight forward method that we use for whole mount staining of the murine retinal vascular …
(PDF) Optimized Protocol for Retinal Wholemount
WEBDec 31, 2013 — In this chapter, we review the main techniques for detecting the total population of RGCs and various of their subtypes in whole-mounted retinas of pigmented and albino rats and mice, four of...
Cell Press: STAR Protocols
WEBJun 2, 2022 — Here, we document detailed whole-mount procedures for immunolabeling the adult retina, enabling high-quality studies of fluorescent-tagged targets with straightforward preparations.
The Retinal Wholemount Technique: A Window to …
WEBJan 16, 2012 — The present study examines all aspects of the wholemount technique, including enucleation, fixation, retinal extraction, flattening, staining, visualization of labelled cells and stereological mapping of cell …
Whole mount immunofluorescent staining of the neonatal mouse …
WEBJul 9, 2013 — By analyzing the retinal vascular plexus at different times after birth, it is possible to observe the various stages of angiogenesis under the microscope. This …
Immunostaining of Whole-Mount Retinas with the …
WEBMar 6, 2021 — Immunostaining needs to be carried out in whole-mount retinas to reveal three-dimensional branching of DACs and the presence of glutamate receptors on subcellular compartments.
Optimized Protocol for Retinal Wholemount Preparation for
WEBDec 13, 2013 — The protocol described here is for structural assessment of a wholemount retinal preparation. This includes descriptions of tissue dissection, mounting onto a …
Mouse Retinal Whole Mounts and Quantification of Vasculature …
WEBAug 5, 2015 — This protocol describes the steps involved in the whole mount processing of mouse eyes for visualization of the retinal vasculature. Go to: Materials and Reagents. …
WEB[Abstract] Here we provide a detailed protocol for whole mount staining of mouse retina. This protocol was used to analyze retinal angiogenesis in newborn mice (Sawaguchi et …
Retinal myeloid cells regulate tip cell selection and vascular
WEBJul 5, 2019 — The balance between tip and stalk cells is regulated by endothelial Notch signalling through the expression of Notch ligand Delta-like 4 (Dll4) in tip cells, which …
WEBStain retinal whole-mounts in 96-well plates. Never use less than 100 µl for staining steps and ensure immersion after solution changes. For washes use 200 µl changes, to …
Retinal Cryo-sections, Whole-Mounts, and Hypotonic Isolated …
WEBOct 7, 2018 — We describe retinal microvascular pericyte immunohistochemical staining in cryo-sections, whole-mounts, and hypotonic isolated vasculature using …
Non-separate Mouse Sclerochoroid/RPE/Retina Staining and …
WEBJan 5, 2021 — Here, we describe steps of both non-pigmented and pigmented mouse Sclerochoroid/RPE/Retina whole mount including eyeball preparation, staining, …
WEBWhole-Mount Retina Staining for BrdU DAY 1: Retina dissection and fixation; DNase I and blocking. 1. Dissect eye cups at room temperature in Ringer solution and fix them in …
Dissection and Whole Mount Staining of Retina from Neonatal Mice
WEBOct 5, 2018 — Here we provide a detailed protocol for whole mount staining of mouse retina. This protocol was used to analyze retinal angiogenesis in newborn mice ( …
Whole Mount Immunofluorescent Staining of the Neonatal Mouse …
WEBJul 9, 2013 — Summary. The neonatal murine retina provides a well characterized physiological model of angiogenesis, which permits investigations of the roles of different …
WEBWhole-mount fluorescence immunohistochemistry is a primary method of microscopy-based phenotypic analysis in the zebrafish model system. In this study, fluorescence …
WEB(A) In current protocols, whole mount staining is performed with separated tissues of RPE and retina, which damages the subretinal region (indicated by red Xs) between RPE …
Whole Mount Immunofluorescent Staining of the Neonatal Mouse …
WEBHere we provide a detailed description of a reliable and technically straight forward method that we use for whole mount staining of the murine retinal vascular plexus, from the …
A New Method to Visualize the Intact Subretina From Retinal …
WEBFeb 16, 2016 — An RPE/retina whole mount staining protocol provided better morphology of the photoreceptor outer segment than current retina whole mount. For the …
The IL-33/ST2 Axis Protects Retinal Ganglion Cells by ... - Springer
WEB2 days ago — The retinal flat-mount was imaged using confocal microscopy. βIII-tubulin was also used as a marker for retinal nerve fibers to quantify the surviving ... C …
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