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PRO-seq: Precise Mapping of Engaged RNA Pol II at Single
Precision run-on sequencing (PRO-seq) maps the position of engaged RNAPII complexes at single-nucleotide resolution and can reveal direct targets of regulation, locations of enhancers, …
PRO‐seq: Precise Mapping of Engaged RNA Pol II at …
Dec 27, 2023 · Precision run-on sequencing (PRO-seq) maps the position of engaged RNAPII complexes at single-nucleotide resolution and can reveal direct targets of regulation, locations …
PRO-seq: Precise Mapping of Engaged RNA Pol II at Single …
Precision run-on sequencing (PRO-seq) maps the position of engaged RNAPII complexes at single-nucleotide resolution and can reveal direct targets of regulation, locations of enhancers, …
Precision run-on sequenc-ing (PRO-seq) maps the position of engaged RNAPII complexes at single-nucleotide resolution and can reveal direct targets of regulation, locations of enhancers, …
Precise Maps of RNA Polymerase Reveal How Promoters Direct …
Feb 22, 2013 · We developed a genome-wide, nuclear run-on assay called PRO-seq that has the sensitivity of GRO-seq but maps Pol II with base pair resolution. PRO-seq uses biotin-labeled …
Base-pair-resolution genome-wide mapping of active RNA
Jul 21, 2016 · Mahat et al. describe how to map the genome-wide positions of active RNA polymerases using a modified nuclear run-on approach called PRO-seq. Details for PRO-cap, …
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PRO-IP-seq tracks molecular modifications of engaged Pol II
Nov 3, 2023 · Here, we develop Precision Run-On coupled to Immuno-Precipitation sequencing (PRO-IP-seq), which double-selects nascent RNAs and transcription complexes, and track …
PRO-Seq - Enseqlopedia
Precision nuclear run-on sequencing (PRO-Seq) maps RNAPII pause sites with base-pair resolution during RNA transcription (Kwak et al., 2013) (Mahat et al., 2016). This approach is …
PRO-Seq - Illumina
Precision nuclear run-on sequencing (PRO-Seq) maps RNAP active sites with base-pair resolution. This approach is similar to GRO-Seq, but it provides the added benefit of single …
PGC-1α senses the CBC of pre-mRNA to dictate the fate of …
Jan 19, 2023 · Our integrated analyses of PRO-seq and RIP-seq experiments demonstrate, genome-wide, that the PGC-1α CBM promotes the release of RNAPII pausing at genes whose pre-mRNAs it binds, i.e., that PGC-1α regulates these genes directly.
PRO‐seq: Precise Mapping of Engaged RNA Pol II at
Dec 27, 2023 · Precision run‐on sequencing (PRO‐seq) maps the position of engaged RNAPII complexes at single‐nucleotide resolution and can reveal direct targets of regulation, locations …
U1 snRNP increases RNA Pol II elongation rate to enable …
We monitored the rate of RNA synthesis using transient transcriptome (TT)-seq, which involves metabolic labeling of RNA during a short pulse of 4sU, followed by selective enrichment of …
Precision run-on sequencing (PRO-seq) for microbiome ... - Nature
Jan 3, 2024 · Precision run-on sequencing (PRO-seq) was initially developed to uncover transient transcriptional signals in eukaryotes 7, 8. PRO-seq captures RNA bound by engaged and...
Distinct patterns of RNA polymerase II and transcriptional …
Dec 27, 2022 · Abe et al. performed ChIL-seq to map Ser2 and Ser5 phosphorylated RNA Pol II forms in mouse embryos before, during, and after major ZGA. Functional perturbations and …
Integrator endonuclease drives promoter-proximal termination at …
Nov 17, 2022 · Heatmaps reflecting the difference in PRO-seq signal upon PROTAC treatment reveal a widespread increase in RNAPII elongating into lncRNA genes (Figure 3 E). The …
Global identification of SWI/SNF targets reveals ... - Cell Press
Nov 2, 2023 · Analysis of PRO-seq data showed that cluster 1 genes displayed lower occupancy by engaged RNAPII in both sense and antisense directions (Figure 4C), as well as lower …
Article U1 snRNP increases RNA Pol II elongation rate to enable ...
Apr 20, 2023 · PRO-seq and RNAPII ChIP-seq signal increase significantly within GC-rich exons as compared with the surrounding AT-rich introns, indicative of slower elongation in exons …
Causes and consequences of RNA polymerase II stalling during …
Nov 18, 2020 · The journey of RNA polymerase II (Pol II) as it transcribes a gene is anything but a smooth ride. Transcript elongation is discontinuous and can be perturbed by intrinsic …
PRO-Seq - Illumina, Inc.
Precision nuclear run-on sequencing (PRO-Seq) maps RNAP active sites with base-pair resolution. This approach is similar to GRO-Seq, but it provides the added benefit of single …
TFIID Enables RNA Polymerase II Promoter-Proximal Pausing
Instead, the PRO-seq data revealed a sharp reduction in reads downstream of promoter-proximal pause sites, at around +300 from the TSS in both human and Drosophila cells. These results implicate additional regulatory mechanisms, downstream of …
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