ApplicationsAlthough RNA enzymes were discovered before DNA enzymes, the latter have some distinct advantages. DNA is more cost-effective, and DNA can be made with longer sequence length and can be made with higher … See more
See also• Aptamer – Oligonucleotide or peptide molecules that bind specific targets
• Ribozyme – Type of RNA molecules
• Systematic evolution of ligands by exponential enrichment (SELEX) – Technique … See more
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DNAzymes: from creation in vitro to application in vivo
DNAzymes are generated de novo by in vitro selection--a powerful and yet simple technique that has been routinely used to isolate extremely rare DNA or RNA sequences with a function of …
- Author: J. C. Achenbach, W. Chiuman, R. P.G. Cruz, Y. Li
- Publish Year: 2004
DNAmoreDB - A Database of Deoxyribozymes
DNAmoreDB is a comprehensive resource of DNAzymes, single-stranded DNA molecules that can perform catalysis. It provides sequence, structure, reaction, and publication information of DNAzymes isolated by in vitro selection and …
DNA Catalysis: The Chemical Repertoire of …
Deoxyribozymes or DNAzymes are single-stranded catalytic DNA molecules that are obtained by combinatorial in vitro selection methods.
DNAmoreDB, a database of DNAzymes - PMC
DNAzymes, also known as deoxyribozymes, are synthetic single-stranded DNA molecules able to catalyze chemical reactions.
DNAzymes: Expanding the Potential of Nucleic Acid Therapeutics
In this study we provide an overview of an emerging class of nucleic acid therapeutic, called DNAzymes. In particular, we review the use of chemical modifications and carrier molecules …
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The dynamic world of the 8–17 DNAzyme - Nature
Jun 17, 2024 · High-precision DNA-based biocatalysts, known as DNA enzymes or DNAzymes, are experiencing a revival in biotechnological, analytical, and therapeutical applications.
Designing in vivo active DNAzymes | Nature Chemistry
Mar 30, 2021 · Now, a promising approach for constructing DNAzymes that show high gene-silencing efficiency in mammalian cells has been developed. This approach incorporates …
Therapeutic DNAzymes: From Structure Design to …
Therapeutic DNAzymes have unceasingly intrigued the scientific community owing to their prosperous gene regulation capability. The efficacy of DNAzymes against many types of diseases has been extensively studied for over two …
Single–Molecule Study of DNAzyme Reveals …
Jan 7, 2023 · DNAzyme is a class of DNA molecules that can perform catalytic functions with high selectivity towards specific metal ions. Due to its potential applications for biosensors and medical therapeutics, DNAzyme has been …
DNAzymes – How Active DNA Biocatalysts That …
Dec 29, 2021 · DNAzymes – a word made up of DNA and enzyme – are catalytically active DNA sequences. They comprise a catalytic core comprising around 15 nucleic acids flanked by short binding arms on the right- and left …
A biologically stable DNAzyme that efficiently silences gene
Mar 25, 2021 · Here we report a re-engineered version of the classic 10–23 DNAzyme that mediates persistent gene-silencing activity in cultured mammalian cells and simultaneously …
DNAzymes: Synthetic Enzymes Made of DNA | SpringerLink
Sep 4, 2019 · Learn about DNAzymes, man-made single-stranded DNA molecules with catalytic abilities, derived by in vitro selection. Focus on RNA-cleaving DNAzymes, their properties, …
Evidence of a General Acid/Base Catalysis Mechanism in the 8 …
Mar 3, 2018 · DNAzymes or deoxyribozymes are short single-stranded DNA molecules with catalytic properties. 1 – 7 Unlike naturally occurring ribozymes, DNAzymes have been …
how active DNA molecules with therapeutic potential work
Dec 22, 2021 · DNAzymes -- a word made up of DNA and enzyme -- are catalytically active DNA sequences. They comprise a catalytic core comprising around 15 nucleic acids flanked by …
DNAzymes, Novel Therapeutic Agents in Cancer Therapy: A …
Gene-specific catalytic DNA molecules, or DNAzymes, have shown promise as a therapeutic intervention against cancer in various in vitro and in vivo models, expediting towards clinical …
Multivalent DNAzyme nanomachine generated by exonuclease III …
Exo III-assisted multivalent DNAzyme nanomachine for rapid, ultrasensitive electrochemical biosensing. The assay enables the detection of HBV DNA down to 45 aM. The system …
Dual-functional DNAzyme powered CRISPR-Cas12a sensor for …
Feb 10, 2024 · Dual-functional DNAzyme was designed to power CRISPR-Cas12a sensor for Pb 2+ detection. DNAzyme/AuNP probe was fabricated to offer one-to-multiple CRISPR-Cas12a …
Biosensing with DNAzymes - PMC
In Section 3, we will discuss the development of DNAzymes for sensing applications, with a particular focus on various DNAzyme-utilizing strategies designed for the detection of metal …
DNAzyme-mediated CRISPR/Cas12a bioassay for label-free …
2 days ago · In our method, the substrate strand of Cu 2+-responsive DNAzyme serves as an activator, binding to the Cas12a-crRNA duplex to trigger the cleavage of G4 DNA by activated …
