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    Comparative stages of embryonic development and chick quality at hatch for Non-SPIDES & SPIDES treated eggs after prolonged storage according to the procedures of Hamburger-Hamilton (1951) stages. KEY: Plates a, c, e, g, i (orange-arrows transition: Non-SPIDES or control treatments); Plates b, d, f, h, j (blue-arrows transition: SPIDES treatments); (a) Unincubated non-SPIDES egg. Blastoderm under-developed with unclear 'donut appearance'. (b) Unincubated SPIDES egg. Blastoderm is well-developed with clear 'donut-appearance'. (c) 'donut-shape' enlarged with no appearance of 'blood islet' (d) 'donut-shape' enlarged greatly with the appearance of 'blood islets' (e) 'donut-shape' enlarged greatly with only 'blood islets' in the outer ring of developing embryo. No appearance of the circulatory system and embryonic appendages at this stage (f) Clear appearance of an embryo with a well-developed circulatory system and embryonic appendage. (g) Protrated terminal hatching with few externally pipped chicks (h) rapid terminal hatching with more hatched and externally pipped chicks. (i) Poor quality chick (light, weak and passive) (j) good quality chick (heavy, thrifty, and active).
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    The main functions of the ovaries are:
    • Producing eggs (oocytes) for fertilization.
    • Producing sex steroid hormones such as estrogen and progesterone.
    • Releasing eggs during ovulation.
    • Making hormones including estrogen, progesterone, and testosterone.
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