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Whole-mount staining of organoids can be used for antibody based characterization. In this organoid staining protocol we detail methods to fix, permeabilize, and stain whole-mount …
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Apr 27, 2020 · By using CUBIC-HistoVIsion for 3D staining, tissue clearing, and volumetric imaging, we successfully stained and imaged whole adult …
- Author: Etsuo A. Susaki, Chika Shimizu, Akihiro Kuno, Akihiro Kuno, Kazuki Tainaka, Xiang Li, Kengo Nishi, K...
- Publish Year: 2020
Whole-mount skeletal staining permits evaluation of the shapes and sizes of skeletal elements in their appropriate locations. The technique is thus the major method for detecting changes in …
- Author: Diana Rigueur, Karen M. Lyons
- DOI: 10.1007/978-1-62703-989-5_9
- Publish Year: 2014
- Published: 2014
The advantage of using fluorescence to stain whole mount sections is that confocal microscopy can be used to section through the larger embryo or tissue sample without having to manually …
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Mar 3, 2022 · Here we describe an optimized protocol for X-gal staining of tissue clearing embryo and adult mouse using CUBIC. The activity of LacZ knock-in reflecting endogenous …
Sep 19, 2020 · Whole-mount staining preserves the three-dimensional spatial information in biological samples allowing a comprehensive interpretation of expression domains.
Oct 28, 2015 · In plants robust and reliable techniques are highly required for the accurate whole-mount visualization of subcellular protein localization in relatively thick specimens, in a well …
May 7, 2013 · Whole-mount immunofluorescent staining facilitates the profiling of protein expression patterns within diverse and complex tissues.
Feb 9, 2012 · In this protocol we describe the whole-mount fixation and multimarker staining procedure, the tissue transparency treatment, microscopy and the analysis of resulting images.
May 10, 2018 · Whole-mount bone staining has commonly been used to examine the morphology of skeletal system, especially for reproductive toxicology testing. Since the original …
Organ Culture and Whole Mount Immunofluorescence Staining of …
Jan 13, 2017 · In this study, we describe an in vitro culture system and whole mount immunofluorescence protocol to better visualize the process of WD coiling and development, …
Whole-Mount Immunofluorescence Staining of Early Mouse …
Immunofluorescence staining enables the visualization of protein expression at a cellular or even sub-nuclear level. Whole-mount staining preserves the three-dimensional spatial information in …
For whole mount and confocal imaging, the tissue needs to be thin or deformable, such as the diaphragm, spinotrapezius, or fat. The tissue can be stored for a few days in the refrigerator. …
Immunohistochemistry of Whole-Mount Mouse Embryos - CSH …
The technique presented this protocol is ideal for obtaining an overall, three-dimensional picture of the distribution of an antigen in embryos from the prestreak stage to ~10.5 dpc (days post …
Cell Press: STAR Protocols
Jan 20, 2022 · Whole mount adipose tissue processing for immunofluorescence staining and imaging; Clearing-free protocol that uses Z-depth reduction and autofluorescence quenching; …
ICC Staining Protocol for Monolayer or Air-Liquid Interface
Below, we describe a protocol for whole-mount ICC staining of ALI cultures derived from any tissue type or monolayer cultures (derived from primary cells, pluripotent stem cells, or cell …
Fluorescence-based Single-cell Analysis of Whole-mount-stained …
Jun 20, 2021 · Here, we present a simple protocol for combined whole-mount immunostaining, tissue-clearing, and optical analysis of large-scale (approx. 1 mm) 3D tissues with single-cell …
Tissue clearing and imaging methods for cardiovascular …
Apr 4, 2021 · Whole-mount immunohistochemical staining followed by clearing with BABB was used to visualize 3D myocardial or vascular architecture (Ivins et al., 2016; Miller et al., 2005). …
Whole-Mount Immunofluorescence Staining, Confocal Imaging …
Dec 22, 2020 · Whole-mount immunofluorescence staining is intended for smaller sections of tissue without the need for manual sectioning. To this purpose, the mouse heart is dissected, …
Whole-Mount Immunofluorescence Staining of Plant Cells and …
Jan 1, 2015 · In this chapter, a whole-mount immunofluorescent staining method of cells and tissues is detailed. Immunostaining using fluorescence-labeled antibodies remains an …
Improved whole-mount immunofluorescence protocol for …
2 days ago · Energy storage and endocrine functions of the Drosophila fat body make it an excellent model for elucidating mechanisms that underlie physiological and pathophysiological …
Mouse Mammary Gland Whole Mount Preparation and Analysis
Jul 7, 2018 · Whole mounts provide this information, are inexpensive and do not require specialized equipment. This protocol describes mammary gland isolation, whole mount …
Pipeline described here is a simple, highly sensitive, multiplexed immunohistochemistry chromogen-based staining on a single slide method, which is called MICSSS, to …
Whole-mount immunofluorescence staining of blood and …
Jun 6, 2023 · Here, we present a protocol for whole-mount immunofluorescence staining of blood and lymphatic vessels in the adult mouse small intestine. We describe the steps for …
Human iPSC–liver organoid transplantation reduces fibrosis …
1 day ago · Bottom: Dipeptidyl peptidase IV (DPP IV) staining (red). (E) Quantification of the tissue volume, surface area, and cross-sectional area in the fetal liver and graft after Tx (n = 3 …
A novel protocol of whole mount electro-immunofluorescence …
Mar 6, 2009 · These results indicate that the whole mount electro-immunofluorescent staining allows the detection of antigens in all layers of cornea, i.e., epithelium, stroma, and …
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