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Jun 23, 2012 · The difference in energy between the original state and this new state leads to a shift in the emitted photon's frequency away from the excitation wavelength. The Raman effect, which is a light scattering phenomenon, should not be confused with absorption (as with fluorescence) where the molecule is excited to a discrete (not virtual) energy level.

Feb 28, 2010 · Luminescence, as I understand it, involves specific transitions between bound states. Fluorescence, for example. Phosphorescence (the reason for the broad-band output of fluorescent lights) involves forbidden transitions- they are …

Jun 6, 2016 · As I know the most common geometry used for fluorescence is right angle observation. Detector is placed at 90 deg to incident light to minimize the risk of transmitted or reflected incident light reaching the detector (from wiki). Why?!

Aug 22, 2022 · Fluorescence and phosphorescence are two categories of luminescence; the difference between the two depends on the nature of the excited state. If the excited state is a singlet state, the return to ground state is 'allowed' (selection rules) and the fluorescence lifetime is very short- 10 ns.

Mar 16, 2013 · I have taken a time dependent fluorescence series of spectra for an ethylene glycol solution using a 532nm laser as the excitation source. I have attached the spectra to this post. Nothing looks unusual-there is a sharp notch near the 532nm line--this is due to a filter I have used to protect...

May 20, 2016 · The absorbing wavelengths take fluorescence away from the overlapping region, while the re-emitted fluorescence covers the complete fluorescence spectrum. Emission spectrum independence of the excitation wavelength: the shape of the fluorescent emission spectrum is independent of the excitation wavelength, correct?

Jun 7, 2021 · When working with a pulsed laser (nitrogen), How can the fluorescence lifetime be longer than the pulse of the laser? Thanks for any explanations

Dec 16, 2013 · I need help understanding the relationship between the excitation and emission spectra for a fluorescent material. This is not homework. Let's say we have the following graph which plots the two spectra for a given material: Two curves are shown - …

Feb 7, 2010 · To obtain the excitation spectrum, you expose the material with radiation, usually just a narrow range of energies, and measure the fluorescence at > 90 degrees from the direction of incidence with a detector that can simultaneously measure and sort photons of different energies. This result is an excitation spectrum.

Dec 9, 2024 · Hello everyone, I’m working on a project where I need to use a cylindrical tube with a vacuum pump to suck in mechanical parts. The tube approaches the part, and the vacuum generated by the pump draws the part into the tube. I want to understand how to link the key parameters of the system: the...