SFC has been used primarily for separation of chiral molecules, mainly those which required normal phase conditions. … See more

Instrumentation of supercritical fluid chromatography SFC has a similar setup to an HPLC instrument. The stationary phases are similar, and are packed inside similar column types. However, there are special features in t… See more

A rule-of-thumb is that any molecule that will dissolve in methanol or a less polar solvent is compatible with SFC, including non-volatile polar solutes. CO2 has polarity similar to n-heptane at its critical point. The solvent's el… See more

The mobile phase is composed primarily of supercritical carbon dioxide, but since CO2 on its own is too non-polar to effectively elute many analytes, cosolvents are added to modify the mobile phase polarity. Cosolvent… See more

There have been a few technical issues that have limited adoption of SFC technology in the past. First of all, is the need to keep a high gas pressure in the operating conditions. High-pressure vessels are expensi… See more