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- Immunofluorescence staining enables the visualization of protein expression at a cellular or even sub-nuclear level. Whole-mount staining preserves the three-dimensional spatial information in biological samples allowing a comprehensive interpretation of expression domains.
Author: Frederick C K WongPublish Year: 2021pubmed.ncbi.nlm.nih.gov/32944908/ - People also ask
Protocol Guide: Immunofluorescent Staining of Whole-Mount …
Whole-mount staining of organoids can be used for antibody based characterization. In this organoid staining protocol we detail methods to fix, permeabilize, and stain whole-mount …
IHC staining protocol for whole mount samples | abcam
May 18, 2023 — Protocol and tips for whole mount immunohistochemical staining. Whole mount staining is the staining of small pieces of tissue – usually embryos – without sectioning. …
- Studies of whole mount immunofluorescence
The advantage of using fluorescence to stain whole mount sections is that confocal microscopy can be used to section through the larger embryo or tissue sample without having to manually …
Whole-Mount Immunofluorescence Staining of Early Mouse …
Sep 19, 2020 — Immunofluorescence staining enables the visualization of protein expression at a cellular or even sub-nuclear level. Whole-mount staining preserves the three-dimensional …
Antigen retrieval and clearing for whole-organ …
Apr 8, 2020 — Here, we describe a detailed protocol for FLASH (fast light-microscopic analysis of antibody-stained whole organs) 1, 2, a versatile approach for whole-organ molecular labeling and...
Combined whole-mount fluorescence in situ hybridization and
Sep 9, 2020 — This protocol describes a combined approach for whole-mount fluorescence in situ hybridization (FISH) and immunofluorescence staining in zebrafish embryos and larvae.
Whole-Mount Immunofluorescence Staining of Early Mouse …
Immunofluorescence staining enables the visualization of protein expression at a cellular or even sub-nuclear level. Whole-mount staining preserves the three-dimensional spatial …
Protocol for whole-mount immunofluorescence staining of ECM …
Jun 21, 2024 — Here, we present a protocol for whole-mount immunofluorescence staining of gel-embedded pancreatic organoids. We describe steps for sample fixation, blocking, and …
Whole-mount immunofluorescence staining of blood and …
Jun 6, 2023 — Here, we present a protocol for whole-mount immunofluorescence staining of blood and lymphatic vessels in the adult mouse small intestine. We describe the steps for perfusion …
A Whole-mount Immunofluorescence Protocol for Three
May 7, 2013 — Whole-mount immunofluorescence is one useful assay to address which signalling molecules are expressed during organ development and up- or down-regulated in …
High-resolution 3D imaging of fixed and cleared organoids
May 3, 2019 — In addition to visualization of whole-mount organoids at cellular resolution, we combined organoid 3D imaging with the Airyscan Fast imaging module of the Zeiss LSM 880 …
Whole-Mount Immunohistochemical and Immunofluorescence …
Jan 30, 2024 — Immunofluorescence assays are an immunohistochemistry method that uses fluorophores to determine diverse cellular antigens. Zebrafish embryos and larvae, with their …
Painting the Pancreas in Three Dimensions: Whole-Mount
In this chapter, we review a revised whole mount immunofluorescence staining method for studying adult pancreatic islet morphology. This method uses smaller samples and combines …
Whole-Mount Immunofluorescence Staining, Confocal Imaging …
Dec 22, 2020 — Whole-mount immunofluorescence staining is intended for smaller sections of tissue without the need for manual sectioning. To this purpose, the mouse heart is dissected, …
Whole-Mount Immunofluorescence Staining, Confocal Imaging …
Dec 22, 2020 — Whole-mount immunofluorescence staining is intended for smaller sections of tissue without the need for manual sectioning. To this purpose, the mouse heart is dissected, …
Whole-mount fluorescence immunohistochemistry is a primary method of microscopy-based phenotypic analysis in the zebrafish model system. In this study, fluorescence …
Improved whole-mount immunofluorescence protocol for ... - bioRxiv
Apr 15, 2024 — Overall, we provide several recommendations for steps in a whole mount staining protocol that results in consistent and robust immunofluorescence labeling of the adult …
Whole-mount immunofluorescence staining of mesenchymal
Jul 31, 2022 — Here, we describe the detailed protocol for whole-mount immunofluorescence staining of mesenchymal progenitors in mouse plantaris muscle. Z-stack image …
Whole-mount Immunofluorescence Imaging: A Fixing and …
Whole-mount imaging allows the study of intact 3D organoids while maintaining their morphology and heterogeneity. To begin staining, treat the organoids with a suitable fixative to …
Cell Press: STAR Protocols
May 18, 2023 — Here, we present a protocol for whole-mount immunofluorescence staining of blood and lymphatic vessels in the adult mouse small intestine. We describe the steps for …
An Optimized Whole-Mount Immunofluorescence Method for …
We present the whole-mount immunofluorescence procedure using maize ear primordia and Arabidopsis inflorescence apices as examples, followed by tips and suggestions for each step. …
Whole mount Immunofluorescence for C. elegans - Greer Lab
Whole mount Immunofluorescence for C. elegans. General Whole mount ICC Protocol for Worms L16-Adult. Remember to have a negative control which is not exposed to primary …
Whole-mount immunofluorescence staining of blood and
Jun 16, 2023 — Here, we present a protocol for whole-mount immunofluorescence staining of blood and lymphatic vessels in the adult mouse small intestine. We describe the steps for …
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