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  2. Whole mount staining is the staining of small pieces of tissue – usually embryos – without sectioning. Whole mount staining is very similar to immunocytochemistry (ICC) or staining of cryosections.

    IHC staining protocol for whole mount samples - Abcam

    www.abcam.com/en-us/technical-resources/protocols/wholemount-staining-for-ihc
    www.abcam.com/en-us/technical-resources/protocols/wholemount-staining-for-ihc
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  3. People also ask
    How can whole-mount staining improve adipose tissue anatomy?
    To overcome this limitation, whole-mount staining can be further combined with tissue clearing techniques to remove the opaqueness of tissue and allow for complete visualization of entire adipose tissue anatomy using light-sheet fluorescent microscopy.

    Visualization of 3D White Adipose Tissue Structure Using Whole-moun…

    pubmed.ncbi.nlm.nih.gov
    Why is whole-mount staining important?
    Hence, the structures of adipocytes and other cellular components are maintained without distortion, achieving the most representative 3D visualization of the tissue. In addition, whole-mount staining can be combined with lineage tracing methods to determine cell fate decisions.

    Visualization of 3D White Adipose Tissue Structure Using Whole-moun…

    pubmed.ncbi.nlm.nih.gov
    Can X-gal staining be used as a whole-mount stain?
    X-gal staining is not guaranteed to work for cells that reside deeply within the tissues when using this protocol for whole-mount staining. It is recommended to perform X-gal staining on isolated organs in parallel.

    Protocol for whole-mount X-gal staining combined with tissue clearing

    sciencedirect.com
    Can whole-mount staining be used to determine cell fate?
    In addition, whole-mount staining can be combined with lineage tracing methods to determine cell fate decisions. However, this technique has some limitations to providing accurate information regarding deeper parts of adipose tissue.

    Visualization of 3D White Adipose Tissue Structure Using Whole-moun…

    pubmed.ncbi.nlm.nih.gov
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  4. Abcam
    https://www.abcam.com/.../wholemount-staining-for-ihc

    IHC staining protocol for whole mount samples - Abcam

    • Whole mount

      The advantage of using fluorescence to stain whole mount sections is that …

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    • Nature
      https://www.nature.com/articles/s41467-0…

      Versatile whole-organ/body staining and imaging …

      WEBApr 27, 2020 · By using CUBIC-HistoVIsion for 3D staining, tissue clearing, and volumetric imaging, we successfully stained and imaged whole adult mouse brains, a whole adult marmoset brain hemisphere,...

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      Publish Year:2020
      Tissue Clearing Brain
      Tissue Clearing and Imaging
    • Studies of whole mount tissue staining
      National Center for Biotechnology Information
      Ten Approaches That Improve Immunostaining: A Review of the Latest Advances for the Optimization of Immunofluorescence
      Immunostaining has emerged as one of the most common and valuable techniques that allow the localization of proteins at a quantitative level within cells and tissues using antibodies coupled to enzymes, fluorochromes, or colloidal nanogold particles. The application of fluorochromes during immunolab...
      Author: Ricardo Piña, …
      Published: 2022/02
      Publication: Int J Mol …
      National Center for Biotechnology Information
      Whole-Mount Skeletal Staining - PMC - National Center for Biotechnology ...
      The first step in almost every investigation of skeletal phenotypes is analysis of whole-mount skeletal preparations. Whole-mount skeletal staining permits evaluation of the shapes and sizes of skeletal elements in their appropriate locations. The technique is thus the major method for detecting cha...
      Author: Diana Rigueur…
      Published: 2014
      DOI: 10.1007/978-1-62…
      PubMed
      Whole-Mount Immunofluorescence Staining of Early Mouse Embryos
      Immunofluorescence staining enables the visualization of protein expression at a cellular or even sub-nuclear level. Whole-mount staining preserves the three-dimensional spatial information in biological samples allowing a comprehensive interpretation of expression domains. Here we describe the samp...
      Author: Frederick C K …
      Published: 2021
      Keywords: Embryo; Im…
      National Center for Biotechnology Information
      Mouse Mammary Gland Whole Mount Preparation and Analysis
      The mammary gland undergoes extensive remodeling during pregnancy and is also subject to neoplastic processes both of which result in histological changes of the gland epithelial structure. Since the mammary tree is a complex three-dimensional structure a method is needed that provides an overview o...
      Author: Cornelia Tolg, …
      Published: 2018/07/07
      Publication: Bio Proto…
      PubMed
      iDISCO: a simple, rapid method to immunolabel large tissue samples for volume imaging
      The visualization of molecularly labeled structures within large intact tissues in three dimensions is an area of intense focus. We describe a simple, rapid, and inexpensive method, iDISCO, that permits whole-mount immunolabeling with volume imaging of large cleared samples ranging from perinatal mo...
      Author: Nicolas Renier
      Published: 2014/11/06
      Keywords: National In…
      PubMed
      Fluorescence-based Single-cell Analysis of Whole-mount-stained and Cleared Microtissues and Organoids for High Throughput Screening
      Three-dimensional (3D) cell culture, especially in the form of organ-like microtissues ("organoids"), has emerged as a novel tool potentially mimicking human tissue biology more closely than standard two-dimensional culture. Typically, tissue sectioning is the standard method for immunohistochemical...
      Author: Henrik Renner
      Published: 2021/06/20
      Keywords: (High-conte…
      National Center for Biotechnology Information
      Tissue clearing and imaging methods for cardiovascular development
      Recent advances in tissue clearing technology have enabled rapid and comprehensive cellular analyses of entire organs in the whole-mount preparations by a combination of state-of-the-art technologies of optical imaging and image processing. All tissue clearing methods seek to homogenize the refracti...
      Author: Hana Kolesov…
      Published: 2021/04/04
      Publication: iScience. …
      PubMed
      Visualization of 3D White Adipose Tissue Structure Using Whole-mount Staining
      Adipose tissue is an important metabolic organ with high plasticity and is responsive to environmental stimuli and nutrient status. As such, various techniques have been developed to study the morphology and biology of adipose tissue. However, conventional visualization methods are limited to studyi...
      Author: Yanqing Jiang
      Published: 2018/11/17
      Keywords: National In…
      National Center for Biotechnology Information
      Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry
      Working with delicate tissue can be a complicating factor when performing immunohistochemical assessment. Here, we present a method that utilizes a ring-supported hydrophilized PTFE membrane to provide structural support to both living and fixed tissue during immunohistochemical processing, which al...
      Author: Elena Ivanova…
      Published: 2013
      Publication: J Vis Exp…
      PubMed
      Whole-Mount Immunofluorescence Staining, Confocal Imaging and 3D Reconstruction of the Sinoatrial and Atrioventricular Node in the Mouse
      The electrical signal physiologically generated by pacemaker cells in the sinoatrial node (SAN) is conducted through the conduction system, which includes the atrioventricular node (AVN), to allow excitation and contraction of the whole heart. Any dysfunction of either SAN or AVN results in arrhythm...
      Author: Ruibing Xia
      Published: 2020/12/22
      Keywords: National In…
      PubMed
      Whole-Mount Imaging of Adipose Tissue Macrophages
      The adipose tissue comprises highly heterogeneous macrophage populations, which play critical roles in the regulation of adipose tissue function and dysfunction during health and disease. Whole-amount staining is a powerful technique for macrophage characterization within the 3D environment of the a...
      Author: Lydia Sorokin
      Published: 2024
      Keywords: Adipose tis…
    • MilliporeSigma
      https://www.sigmaaldrich.com/US/en/technical...

      Protocol Guide: Immunofluorescent Staining of Whole-Mount …

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      Zoids: Chaotic Century
      Confocal Microscopy Organoids Protocol
      Mount, Cornwall
    • Nature
      https://www.nature.com/articles/s41596-019-0160-8

      High-resolution 3D imaging of fixed and cleared organoids

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      Confocal Microscopy Organoids Protocol
      3d Organoids
      3d Cell Culture Organoid
    • abcam.com
      https://docs.abcam.com/pdf/protocols/Whole_mount_fluorescent_ihc.pdf
      [PDF]

      Whole mount - Abcam

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      Tissue
      Whole Mount Embryo Stain

    • Images of Whole mount tissue staining

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    • National Center for Biotechnology Information
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8836139

      Ten Approaches That Improve Immunostaining: A Review of the …

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      Presidential Memorial Certificate
      Published:2022/02
    • National Center for Biotechnology Information
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5384832

      Whole-Mount Skeletal Staining - PMC - National Center for …

      Tags:
      Presidential Memorial Certificate
      Publish Year:2014
      Diana Rigueur, Karen M. Lyons
    • Plant Methods
      https://plantmethods.biomedcentral.co…

      Protocol: an improved and universal procedure for …

      WEBOct 28, 2015 · The protocol is improved and robust for optimal sample fixation, tissue clearing and multi-protein staining procedures and can be used in combination with simultaneous detection of specific …

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      Tissue Clearing Protocol
      Publish Year:2015
    • Springer
      https://link.springer.com/article/10.1007/s10911-013-9285-5

      A Whole-mount Immunofluorescence Protocol for Three

      Tags:
      Publish Year:2013
      Mammary gland
      Naoko Kogata, Beatrice A. Howard
      E 11
    • ScienceDirect
      https://www.sciencedirect.com/science/article/pii/S2666166722000077

      Protocol for whole-mount X-gal staining combined with tissue …

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      Tissue Clearing Protocol
      CUBIC TCP
    • Springer
      https://link.springer.com/protocol/10.1007/978-1-0716-0958-3_10

      Whole-Mount Immunofluorescence Staining of Early Mouse …

      Tags:
      Immunofluorescence Staining
      Publish Year:2021
      Laboratory Techniques in Biology
    • Nature
      https://www.nature.com/articles/nprot.2011.441

      Whole-mount three-dimensional imaging of internally localized ...

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      Whole Mount Staining
      Whole Mount Imaging
      Publish Year:2012
    • ScienceDirect
      https://www.sciencedirect.com/science/article/pii/S2666166723002770

      Whole-mount immunofluorescence staining of blood and …

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      Immunofluorescence Staining
      Tissue
    • PubMed
      https://pubmed.ncbi.nlm.nih.gov/32944908

      Whole-Mount Immunofluorescence Staining of Early Mouse …

      Tags:
      Immunofluorescence Staining
      Publish Year:2021
      Early Mouse Embryos
    • National Center for Biotechnology Information
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8328583

      Mouse Mammary Gland Whole Mount Preparation and Analysis

      Tags:
      Whole Mount Tissue Protocol
      Publish Year:2018
      Mammary gland
    • Cold Spring Harbor Protocols
      https://cshprotocols.cshlp.org/content/2008/1/pdb.prot4820.full

      Immunohistochemistry of Whole-Mount Mouse Embryos - CSH …

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      Alex Joyner
      Embryo
    • PubMed
      https://pubmed.ncbi.nlm.nih.gov/25417164

      iDISCO: a simple, rapid method to immunolabel large tissue

      Tags:
      Publish Year:2014
    • PubMed
      https://pubmed.ncbi.nlm.nih.gov/34262994

      Fluorescence-based Single-cell Analysis of Whole-mount-stained …

      Tags:
      Publish Year:2021
      Zoids: Chaotic Century
    • National Center for Biotechnology Information
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086021

      Tissue clearing and imaging methods for cardiovascular …

      Tags:
      Publish Year:2021
      Tissue Clearing Protocol
      Tissue Clearing Brain
    • PubMed
      https://pubmed.ncbi.nlm.nih.gov/30507917

      Visualization of 3D White Adipose Tissue Structure Using Whole …

      Tags:
      Publish Year:2018
      White County
    • National Center for Biotechnology Information
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4048354

      Optimized Protocol for Retinal Wholemount Preparation for …

      Tags:
      Presidential Memorial Certificate
      Publish Year:2013
      J Vis Exp. 2013; (82): 51018.
    • PubMed
      https://pubmed.ncbi.nlm.nih.gov/33427243

      Whole-Mount Immunofluorescence Staining, Confocal Imaging …

      Tags:
      Immunofluorescence Staining
      Whole Mount Staining
      Whole Mount Tissue Protocol
    • Nature
      https://www.nature.com/articles/s42003-024-06949-1

      Spatial multi-omics in whole skeletal muscle reveals complex …

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      Immunofluorescence Staining
      Coenzyme Q – cytochrome c reductase
    • PubMed
      https://pubmed.ncbi.nlm.nih.gov/37639132

      Whole-Mount Imaging of Adipose Tissue Macrophages

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      Tissue
      Cells