whole mount tissue staining - Search
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  2. The first step in almost every investigation of skeletal phenotypes is analysis of whole-mount skeletal preparations. Whole-mount skeletal staining permits evaluation of the shapes and sizes of skeletal elements in their appropriate locations. The technique is thus the major method for detecting changes in skeletal patterning.
    Author: Diana Rigueur, Karen M. Lyons
    Publish Year: 2014
    DOI: 10.1007/978-1-62703-989-5_9
    Published: 2014

    Whole-Mount Skeletal Staining - National Center for Biotechnolog…

    www.ncbi.nlm.nih.gov/pmc/articles/PMC5384832/
    www.ncbi.nlm.nih.gov/pmc/articles/PMC5384832/
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  3. People also ask
    What is whole-mount bone staining?Whole-mount bone staining has commonly been used to examine the morphology of skeletal system, especially for reproductive toxicology testing. Since the original procedure for whole-mount bone staining was first reported 1, it has undergone various modifications.

    A rapid and nondestructive protocol for whole-mount bone staining of

    nature.com
    Why do I have granules under my skin with Mast Cell?

    Dr. Anet Varghese

    Doctor of Medicine (MBBS) · 1 years of exp

    Mast cells are resident inflammatory cells found in the dermal layer of the skin. In uninjured tissue, mature mast cells contain abundant intracellular granules (black and gray circles), which are used to store a variety of preformed mediators. During an injury, these mast cells get activated and undergo degranulation.
    Q&A: Mast Cell
    microsoftstart.msn.com
    What is whole-mount staining?Whole-mount staining preserves the three-dimensional spatial information in biological samples allowing a comprehensive interpretation of expression domains. Here we describe the samp … Immunofluorescence staining enables the visualization of protein expression at a cellular or even sub-nuclear level.

    Whole-Mount Immunofluorescence Staining of Early Mouse Embryos

    pubmed.ncbi.nlm.nih.gov
    How long does a whole-mount bone stain take?Scientific Reports 8, Article number: 7453 ( 2018 ) Cite this article Here we propose a new protocol for whole-mount bone staining, which allows the rapid preparation of highly cleared and nondestructive specimens. It only takes 3 days to complete whole procedure for small vertebrates, such as medaka, zebrafish, and Xenopus frogs.

    A rapid and nondestructive protocol for whole-mount bone staining of

    nature.com
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  4. MilliporeSigma
    https://www.sigmaaldrich.com/US/en/technical...

    Protocol Guide: Immunofluorescent Staining of Whole-Mount …

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    Has anyone tried IF staining of intestinal organoids …
    researchgate.net
    Organoid and 3-D Cell Culture Protocols - R&D Systems
    rndsystems.com
    How do you fix organoids for IHC staining and H&E …
    researchgate.net
    High-resolution 3D imaging of fixed and cleared organoids
    nature.com
    Whole-Mount Immunofluorescence Staining, Confocal …
    pubmed.ncbi.nlm.nih.gov
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  5. Nature
    https://www.nature.com/articles/s4146…

    Versatile whole-organ/body staining and imaging …

    WEBApr 27, 2020 · Based on our precise characterization of biological tissues as an electrolyte gel, we experimentally evaluated broad 3D staining …

    • Author: Etsuo A. Susaki, Chika Shimizu, Akihiro Kuno, Akihiro Kuno, Kazuki Tainaka, Xiang Li, Kengo Nishi, K...
    • Publish Year: 2020

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    New staining technique visualizes whole organs and bodies
    sciencedaily.com
    3D Tissue Staining Visualizes Whole Organs - The …
    thepathologist.com
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  6. Studies of whole mount tissue staining
    Whole-Mount Skeletal Staining - National Center for Biotechnology ...
    The first step in almost every investigation of skeletal phenotypes is analysis of whole-mount skeletal preparations. Whole-mount skeletal staining permits evaluation of the shapes and sizes of skeletal elements in their appropriate locations. The technique
    Author: Diana Rigueur, Karen M. Lyons
    Published: 2014
    DOI: 10.1007/978-1-62703-989-5_9
    National Center for Biotechnology Information · nih.gov
    Fluorescence-based Single-cell Analysis of Whole-mount-stained and Cleared Microtissues and Organoids for High Throughput Screening
    Three-dimensional (3D) cell culture, especially in the form of organ-like microtissues ("organoids"), has emerged as a novel tool potentially mimicking human tissue biology more closely than standard two-dimensional culture. Typically, tissue sectioning
    Author: Henrik Renner
    Published: 2021/06/20
    Keywords: (High-content) image analysis; 3D cell culture; Automation; Clearing; Organoids; Whole-mount immunostaining
    PubMed · nih.gov
    iDISCO: a simple, rapid method to immunolabel large tissue samples for volume imaging
    The visualization of molecularly labeled structures within large intact tissues in three dimensions is an area of intense focus. We describe a simple, rapid, and inexpensive method, iDISCO, that permits whole-mount immunolabeling with volume imaging of
    Author: Nicolas Renier
    Published: 2014/11/06
    Keywords: National Institutes of Health; National Center for Biotechnology Information; National Library of Medicine; MEDLINE;
    PubMed · nih.gov
    Protocol for whole-mount X-gal staining combined with tissue clearing in embryo and adult mouse using CUBIC
    Here we describe an optimized protocol for X-gal staining of tissue clearing embryo and adult mouse using CUBIC. The activity of LacZ knock-in reflecting endogenous expression of genes of interest in the whole body can be visualized by X-gal staining.
    Author: Haruko Watanabe-Takano
    Published: 2022/01/20
    Keywords: Developmental biology; Genetics; Microbiology; Model Organisms
    PubMed · nih.gov
    Tissue clearing and imaging methods for cardiovascular development
    Recent advances in tissue clearing technology have enabled rapid and comprehensive cellular analyses of entire organs in the whole-mount preparations by a combination of state-of-the-art technologies of optical imaging and image processing. All tissue
    Author: Hana Kolesová, Veronika Olejníčková, Alena Kvasilová, Martina Gregorovičová, David Sedmera
    Published: 2021/04/04
    Publication: iScience. 2021 Apr 23; 24(4): 102387.
    National Center for Biotechnology Information · nih.gov
    Whole-Mount Immunofluorescence Staining, Confocal Imaging and 3D Reconstruction of the Sinoatrial and Atrioventricular Node in the Mouse
    The electrical signal physiologically generated by pacemaker cells in the sinoatrial node (SAN) is conducted through the conduction system, which includes the atrioventricular node (AVN), to allow excitation and contraction of the whole heart. Any dysfunction
    Author: Ruibing Xia
    Published: 2020/12/22
    Keywords: National Institutes of Health; National Center for Biotechnology Information; National Library of Medicine; MEDLINE;
    PubMed · nih.gov
    Click histochemistry for whole-mount staining of brain structures
    Open in a separate window Method name: Whole-mount click histochemistry Keywords: Click chemistry, 5-ethynyl-2'-deoxyuridine, Whole-mount histochemistry, Neurogenesis, Cell division, Cell migration, Hippocampus, 3D imaging
    Author: Alexander A. Lazutkin, Sergey A. Shuvaev, Natalia V. Barykina
    Published: 2019
    Publication: MethodsX. 2019; 6: 1986–1991.
    National Center for Biotechnology Information · nih.gov
    Visualization of 3D White Adipose Tissue Structure Using Whole-mount Staining
    Adipose tissue is an important metabolic organ with high plasticity and is responsive to environmental stimuli and nutrient status. As such, various techniques have been developed to study the morphology and biology of adipose tissue. However, conventional
    Author: Yanqing Jiang
    Published: 2018/11/17
    Keywords: National Institutes of Health; National Center for Biotechnology Information; National Library of Medicine; MEDLINE;
    PubMed · nih.gov
    Mouse Mammary Gland Whole Mount Preparation and Analysis
    The mammary gland undergoes extensive remodeling during pregnancy and is also subject to neoplastic processes both of which result in histological changes of the gland epithelial structure. Since the mammary tree is a complex three-dimensional structure
    Author: Cornelia Tolg, Mary Cowman, Eva A. Turley
    Published: 2018/07/07
    Publication: Bio Protoc. 2018 Jul 5; 8(13): e2915.
    National Center for Biotechnology Information · nih.gov
    A novel protocol of whole mount electro-immunofluorescence staining
    Purpose To develop a new method of whole mount immunostaining that improves the penetration of staining reagents into the cornea and decreases non-specific binding and background. Methods Adult mouse corneas were fixed overnight in 4% paraformaldehyde
    Author: Hongshan Liu, Winston W.Y. Kao
    Published: 2009
    Publication: Mol Vis. 2009; 15: 505–517.
    National Center for Biotechnology Information · nih.gov
  7. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5384832

    Whole-Mount Skeletal Staining - National Center for …

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    A rapid and nondestructive protocol for whole-mount …
    nature.com
    (PDF) Whole-Mount Skeletal Staining - ResearchGate
    researchgate.net
    Recommended to you based on what's popular • Feedback
  8. abcam.com
    https://docs.abcam.com/pdf/protocols/Whole_mount_fluorescent_ihc.pdf
    [PDF]

    Whole mount - Abcam

  9. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8792446

    Protocol for whole-mount X-gal staining combined with tissue …

  10. Images of Whole mount tissue staining

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  11. Cold Spring Harbor Protocols
    https://cshprotocols.cshlp.org/content/2008/1/pdb.prot4820.full

    Immunohistochemistry of Whole-Mount Mouse Embryos - CSH …

  12. Nature
    https://www.nature.com/articles/nprot.2011.441

    Whole-mount three-dimensional imaging of internally localized

  13. Plant Methods
    https://plantmethods.biomedcentral.com/articles/10...

    Protocol: an improved and universal procedure for whole-mount ...

  14. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10192636

    Whole-mount immunofluorescence staining of blood and …

  15. biologists.com
    https://journals.biologists.com/bio/article/doi/10...

    Improved whole-mount immunofluorescence protocol for …

  16. PubMed
    https://pubmed.ncbi.nlm.nih.gov/34262994

    Fluorescence-based Single-cell Analysis of Whole-mount-stained …

  17. Nature
    https://www.nature.com/articles/s41592-024-02327-1

    Deep 3D histology powered by tissue clearing, omics and AI

  18. Springer
    https://link.springer.com/protocol/10.1007/978-1-0716-0958-3_10

    Whole-Mount Immunofluorescence Staining of Early Mouse …

  19. PubMed
    https://pubmed.ncbi.nlm.nih.gov/25417164

    iDISCO: a simple, rapid method to immunolabel large tissue

  20. Biospecimen Research Database
    https://brd.nci.nih.gov/brd/sop/download-pdf/3284
    [PDF]

    Multiplex IHC Consecutive Staining on a Single Slide …

  21. PubMed
    https://pubmed.ncbi.nlm.nih.gov/35118431

    Protocol for whole-mount X-gal staining combined with tissue

  22. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8086021

    Tissue clearing and imaging methods for cardiovascular …

  23. PubMed
    https://pubmed.ncbi.nlm.nih.gov/33427243

    Whole-Mount Immunofluorescence Staining, Confocal Imaging …

  24. Fralin Biomedical Research Institute at VTC
    https://fbri.vtc.vt.edu/content/dam/fbri_vtc_vt_edu...
    [PDF]

    Microsoft Word - Tissue whole mount immunostaining.docx

  25. Nature
    https://www.nature.com/articles/s41598-018-25836-4

    A rapid and nondestructive protocol for whole-mount bone …

  26. ScienceDirect
    https://www.sciencedirect.com/science/article/pii/S2666166722000077

    Protocol for whole-mount X-gal staining combined with tissue …

  27. Springer
    https://link.springer.com/chapter/10.1007/978-3-319-19944-3_11

    Whole-Mount Immunofluorescence Staining of Plant Cells and …

  28. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6812327

    Click histochemistry for whole-mount staining of brain structures

  29. Science | AAAS
    https://www.science.org/doi/10.1126/scitranslmed.adg0338

    Human iPSC–liver organoid transplantation reduces fibrosis

  30. PubMed
    https://pubmed.ncbi.nlm.nih.gov/30507917

    Visualization of 3D White Adipose Tissue Structure Using Whole …

  31. MilliporeSigma
    https://www.sigmaaldrich.com/.../cytology-stains

    Cytology Stains - MilliporeSigma

  32. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8328583

    Mouse Mammary Gland Whole Mount Preparation and Analysis

  33. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2650717

    A novel protocol of whole mount electro-immunofluorescence …