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- Whole mount staining is a technique used to stain small pieces of tissue, usually embryos, without sectioning12. It is similar to immunocytochemistry (ICC) or staining of cryosections1. Whole-mount immunostaining is intended for small pieces of tissue without sectioning and methods are very similar to immunocytochemistry (ICC) or immunohistochemistry (IHC) staining of cryosections2. Whole-mount staining of organoids can be used for antibody-based characterization2.
Learn more:✕This summary was generated using AI based on multiple online sources. To view the original source information, use the "Learn more" links.Whole mount staining is the staining of small pieces of tissue – usually embryos – without sectioning. Whole mount staining is very similar to immunocytochemistry (ICC) or staining of cryosections.www.abcam.com/protocols/whole-mount-staining-p…Whole-mount immunostaining is intended for small pieces of tissue without sectioning and methods are very similar to immunocytochemistry (ICC) or immunohistochemistry (IHC) staining of cryosections. Whole-mount staining of organoids can be used for antibody based characterization.www.sigmaaldrich.com/US/en/technical-documents… - People also ask
IHC staining protocol for whole mount samples | Abcam
Protocol and troubleshooting tips for whole mount immunohistochemical staining. Whole mount staining is the staining of small pieces of tissue – usually embryos – without sectioning. Whole mount staining is very similar to immunocytochemistry (ICC) or staining of cryosections. See more
Some researchers view and obtain images of embryos as they are. The whole embryo can be imaged while floating in glycerol buffer in a petri dish, before … See more
As the embryo grows, it will become too large to stain. The various reagents, including fixative, antibody and developing solution will not be able to permeate … See more
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Versatile whole-organ/body staining and imaging …
WEBApr 27, 2020 · By using CUBIC-HistoVIsion for 3D staining, tissue clearing, and volumetric imaging, we successfully stained and imaged …
- Author: Etsuo A. Susaki, Chika Shimizu, Akihiro Kuno, Akihiro Kuno, Kazuki Tainaka, Xiang Li, Kengo Nishi, K...
- Publish Year: 2020
- Studies of whole mount tissue staining
Protocol Guide: Immunofluorescent Staining of Whole-Mount …
WEBWhole-mount staining of organoids can be used for antibody based characterization. In this organoid staining protocol we detail methods to fix, permeabilize, and stain whole …
Whole-Mount Skeletal Staining - PMC - National Center for …
WEBWhole-mount skeletal staining permits evaluation of the shapes and sizes of skeletal elements in their appropriate locations. The technique is thus the major method for …
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Whole mount - Abcam
WEBThe advantage of using fluorescence to stain whole mount sections is that confocal microscopy can be used to section through the larger embryo or tissue sample without …
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Protocol: an improved and universal procedure for whole-mount ...
WEBOct 28, 2015 · The protocol is improved and robust for optimal sample fixation, tissue clearing and multi-protein staining procedures and can be used in combination with …
Protocol for whole-mount X-gal staining combined with tissue …
WEBMar 3, 2022 · Here we describe an optimized protocol for X-gal staining of tissue clearing embryo and adult mouse using CUBIC. The activity of LacZ knock-in reflecting …
Whole-Mount Immunofluorescence Staining of Early Mouse …
WEBSep 19, 2020 · Whole-mount staining preserves the three-dimensional spatial information in biological samples allowing a comprehensive interpretation of expression domains.
A rapid and nondestructive protocol for whole-mount bone …
WEBMay 10, 2018 · Whole-mount bone staining has commonly been used to examine the morphology of skeletal system, especially for reproductive toxicology testing. Since the …
Whole-Mount X-Gal Staining of Mouse Tissues - PMC
WEBRemove the desired tissue from the mouse. Clean the tissue with PBS and place on bibulous paper. Cut around the tissue eliminating as much bibulous paper as possible, …
Whole-mount three-dimensional imaging of internally localized ...
WEBFeb 9, 2012 · In this protocol we describe the whole-mount fixation and multimarker staining procedure, the tissue transparency treatment, microscopy and the analysis of …
WEBFor whole mount and confocal imaging, the tissue needs to be thin or deformable, such as the diaphragm, spinotrapezius, or fat. The tissue can be stored for a few days in the …
Immunohistochemistry of Whole-Mount Mouse Embryos - CSH …
WEBImmunohistochemistry of Whole-Mount Mouse Embryos. Alex Joyner and. Nancy Wall. This protocol was adapted from “Techniques for Visualizing Gene Products, Cells, …
iDISCO: a simple, rapid method to immunolabel large tissue
WEBNov 6, 2014 · We describe a simple, rapid, and inexpensive method, iDISCO, that permits whole-mount immunolabeling with volume imaging of large cleared samples ranging …
IHC staining protocol for whole mount samples | abcam
WEBMay 18, 2023 · Protocol and tips for whole mount immunohistochemical staining. Whole mount staining is the staining of small pieces of tissue – usually embryos – without …
A clearing-free protocol for imaging intact whole adipose tissue ...
WEBMar 18, 2022 · Whole mount adipose tissue processing for immunofluorescence staining and imaging. •. Clearing-free protocol that uses Z-depth reduction and autofluorescence …
Protocol for whole-mount X-gal staining combined with tissue …
WEBJan 20, 2022 · Abstract. Here we describe an optimized protocol for X-gal staining of tissue clearing embryo and adult mouse using CUBIC. The activity of LacZ knock-in …
Whole-mount immunofluorescence staining of blood and
WEBJun 16, 2023 · Here, we present a protocol for whole-mount immunofluorescence staining of blood and lymphatic vessels in the adult mouse small intestine. We describe the …
Fluorescence-based Single-cell Analysis of Whole-mount-stained …
WEBJun 20, 2021 · Here, we present a simple protocol for combined whole-mount immunostaining, tissue-clearing, and optical analysis of large-scale (approx. 1 mm) …
High-Speed Clearing and High-Resolution Staining for ... - Springer
WEB2 days ago · Background: Tissue clearing enables deep imaging in various tissues by increasing the transparency of tissues, but there were limitations of immunostaining of …
Visualization of 3D White Adipose Tissue Structure Using Whole …
WEBNov 17, 2018 · Here we present whole-mount staining, an immunohistochemistry method that preserves intact adipose tissue morphology with minimal processing steps. Hence, …
Protocol for whole-mount X-gal staining combined with tissue …
WEBMar 18, 2022 · Summary. Here we describe an optimized protocol for X-gal staining of tissue clearing embryo and adult mouse using CUBIC. The activity of LacZ knock-in …
A versatile tissue-rolling technique for spatial-omics ... - Nature
WEBJun 21, 2024 · This protocol presents a versatile tissue-rolling technique for spatially profiling the transcriptome and proteome of the whole murine gastrointestinal tract with …
Methods in Enzymology (MIE): Methods of Adipose Tissue Biology-
WEBIn this chapter, we will provide detailed protocols for preparing and imaging whole mount, paraffin sectioned and frozen sectioned adipose tissue. We will also provide discussion …
Researchers develop low-cost light sheet fluorescence …
WEB2 days ago · Three-dimensional (3D) imaging of organs and tissues is vital as it can provide important structural information at the cellular level. 3D imaging enables the accurate …
Whole-mount immunofluorescence staining of blood and …
WEBJun 6, 2023 · Here, we present a protocol for whole-mount immunofluorescence staining of blood and lymphatic vessels in the adult mouse small intestine. We describe the steps …
[Imaging for displaying lymphatic vessels in whole-mount aorta
WEBObjective To explore a simple and feasible method for whole-mount immunofluorescence staining of lymphatic vessels in the ApoE-/-mouse model of atherosclerosis. Methods …
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