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  7. People also ask
    How to determine DHRS1 expression in selected human tissues?Two methods were used to determine the DHRS1 expression in selected human tissues - RT-qPCR for absolute quantification of DHRS1 mRNA and western blotting with specific antibodies for the semi-quantitative detection of DHRS1 protein.

    Initial characterization of human DHRS1 (SDR19C1), a member of the

    sciencedirect.com
    What is the sequence alignment of DHRS1 and HSD11B1?Fig. 1. Sequence alignment of human DHRS1 with two well-known human members of SDR cluster 3 – retinoldehydrogenase 10 (RDH10) and 11β-hydroxysteroid dehydrogenase 1 (HSD11B1). Shaded amino acids are involved in the catalytic mechanism.

    Initial characterization of human DHRS1 (SDR19C1), a member of the

    sciencedirect.com
    Does DHRS1 have a broad substrate spectrum?A fairly broad substrate spectrum is a typical feature for the majority of SDR enzymes, such as 11β-HSD1, that is also from the SDR cluster 3 [5, 45 ]. In present study, the expression profile of DHRS1 in human tissues was determined at both the mRNA and protein level.

    Initial characterization of human DHRS1 (SDR19C1), a member of the

    sciencedirect.com
    What is the recombinant form of DHRS1?The recombinant form of DHRS1 was purified using the detergent n-dodecyl-β-D-maltoside, and DHRS1 was proven to be an NADPH-dependent reductase that is able to catalyse the in vitro reductive conversion of some steroids (estrone, androstene-3,17-dione and cortisone), as well as other endogenous substances and xenobiotics.

    Initial characterization of human DHRS1 (SDR19C1), a member of the

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