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  8. People also ask
    How to determine DHRS1 expression in selected human tissues?Two methods were used to determine the DHRS1 expression in selected human tissues - RT-qPCR for absolute quantification of DHRS1 mRNA and western blotting with specific antibodies for the semi-quantitative detection of DHRS1 protein.
    What is the sequence alignment of DHRS1 and HSD11B1?Fig. 1. Sequence alignment of human DHRS1 with two well-known human members of SDR cluster 3 – retinoldehydrogenase 10 (RDH10) and 11β-hydroxysteroid dehydrogenase 1 (HSD11B1). Shaded amino acids are involved in the catalytic mechanism.
    Does DHRS1 have a broad substrate spectrum?A fairly broad substrate spectrum is a typical feature for the majority of SDR enzymes, such as 11β-HSD1, that is also from the SDR cluster 3 [5, 45 ]. In present study, the expression profile of DHRS1 in human tissues was determined at both the mRNA and protein level.
    What is the recombinant form of DHRS1?The recombinant form of DHRS1 was purified using the detergent n-dodecyl-β-D-maltoside, and DHRS1 was proven to be an NADPH-dependent reductase that is able to catalyse the in vitro reductive conversion of some steroids (estrone, androstene-3,17-dione and cortisone), as well as other endogenous substances and xenobiotics.
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