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Methods and Materials: Trituration | Worthington Biochemical
WEBCell Isolation Techniques. Methods and Materials. Working With Enzymes; Basic Primary Cell Isolation; Equilibration with 95% O2-5% CO2; Trituration; Enzymatic Cell …
WEBIn developmental, cell and molecular biology, trituration is the process of fragmenting of solid material (often biological tissue or aggregated material) into smaller components …
- Estimated Reading Time: 3 mins
WEBJan 11, 2007 · Culturing hippocampal neurons. Stefanie Kaech & Gary Banker. Nature Protocols 1 , 2406–2415 ( 2006) Cite this article. 33k …
- Author: Stefanie Kaech, Gary Banker
- Publish Year: 2006
WEBJun 6, 2021 · Summary. This protocol entails a simple method for isolation, culturing, and in vitro differentiation of adult neural stem cells from the dentate gyrus in the hippocampus …
- Author: Ahmed K.M.A. Ahmed, Toke Jost Isaksen, Toshihide Yamashita
- DOI: 10.1016/j.xpro.2021.100522
- Publish Year: 2021
- Publication: STAR Protoc. 2021 Jun 18; 2(2): 100522.
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WEBMay 6, 2021 · This step describes the procedure for brain dissection followed by digestion and trituration of tissue into single cells. It is possible to use this protocol for isolation of …
WEBJun 18, 2021 · Under a Creative Commons license. open access. Highlights. •. A simple protocol for mouse adult neural stem cell isolation and culture. •. Targeted …
WEBMay 22, 2008 · This protocol maintains a very high degree of pluripotent cells partly by mechanical separation of spontaneously differentiating cells. mES cells can be cultured …
WEBMar 7, 2017 · To address this need, we describe here a protocol for the rapid processing of post-mortem autopsy tissue samples in order to generate durable patient-derived cell …
WEBThe cell types that can be cultured include dissociated cultures of neurons, astrocytes, microglia, pericytes and brain endothelial and neural precursor cells, as well as explant …
Mouse Müller Cell Isolation and Culture - PMC - National Center …
WEBAug 5, 2017 · But, mammalian Müller cells still retain certain retinal stem cell properties with various degree of self-renewal and differentiation potentials, and thereby held a …
Cell Press: STAR Protocols
WEBMar 31, 2022 · Gently triturate cells by repeated aspiration and transfer dissociated cells to a 50 mL conical tube containing a greater or equal volume of complete DMEM (Figure …
Techniques for mammalian cell tissue culture - PubMed
WEBAbstract. This unit opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step-by-step protocols describe …
Culturing Human Neural Stem Cells - Thermo Fisher Scientific
WEBIntroduction. Neural stem cells (NSC) are valuable resources because of their ability to differentiate into neurons and glial cells with applications in neuroscience and clinical …
Primary Neurons in Culture and Neuronal Cell Lines for In Vitro ...
WEBJan 1, 2011 · Triturate the cell suspension up to ten times by passage through a 5-mL pipette fitted with a 1,000-μL tip (see Note 10). 6. Transfer cells in a 15-mL conical tube …
Preparation of dissociated mouse cortical neuron cultures
WEBTo increase yields of neuronal cells triturate the pieces of the cortical tissue gently after the enzyme incubation step. This is imperative as it prevents unnecessary injury to cells …
Analysis of microglial migration by a micropipette assay - Nature
WEBJan 30, 2014 · Article 26 July 2021. A tool for mapping microglial morphology, morphOMICs, reveals brain-region and sex-dependent phenotypes. Article Open access 30 September …
Reproducible and scalable differentiation of highly pure cortical ...
WEBJun 16, 2023 · open access. Highlights. •. Generation of highly pure hiPSC-derived cortical neurons by spot-based differentiation. •. Expansion and purification steps to avoid …
Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells
WEBFeb 2, 2016 · In this protocol we describe our modifications to a method to isolate, culture and maintain mouse intestinal stem cells as crypt-villus forming organoids. These cells, …
Cell and Tissue Cultures | SpringerLink
WEB2.1 Definitions and Principles. Cells taken directly from the organism and kept in vitro for more than 24 h are considered to be primary cultures (see Fedoroff, 1977 for tissue …
How to Prepare Single Cells from PSC-Derived Epithelial
WEBBelow are protocols for processing and dissociating PSC-derived epithelial cells cultured in different formats, including as 2D monolayers, monolayers in transwell inserts, and …
Identification and Isolation of Human LGR5+ Cells Using an …
WEBTriturate cells 20× before analysis/sorting with P1000 uncut pipette tip to ensure cells are in a single cell suspension prior to analysis. With unstained control cells, set forward and …
Isolation and Culture of Corneal Stromal Stem Cells
WEBJun 16, 2020 · 3 Citations. Abstract. An increasing body of evidence authenticates the benefit of corneal stroma-derived stem cells (CSSCs) in tissue engineering and …
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